Continuous assay for DNA polymerization by light scattering

K. A. Johnson; F. R. Bryant; S. J. Benkovic

doi:10.1016/0003-2697(84)90324-5

Continuous assay for DNA polymerization by light scattering

K. A. Johnson, F. R. Bryant, S. J. Benkovic

Chemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The time course of DNA polymerization, catalyzed by DNA polymerase I, can be monitored by following changes in light scattering that result from increases in molecular weight of the polymer. Increases in mass of 10% result in an easily measurable change in light scattering. It has been shown that the light scattering change is correlated with the incorporation of radioactive nucleotide, as measured by the filter binding method. Continuous monitoring of the time dependence of polymerization should prove useful in kinetic analysis of the polymerase reaction and related enzyme-DNA interactions.

Original language	English (US)
Pages (from-to)	192-194
Number of pages	3
Journal	Analytical Biochemistry
Volume	136
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(84)90324-5
State	Published - Jan 1984

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0003-2697(84)90324-5

Cite this

@article{5e7509c8344243c3a61c8e0e23b2fa9c,

title = "Continuous assay for DNA polymerization by light scattering",

abstract = "The time course of DNA polymerization, catalyzed by DNA polymerase I, can be monitored by following changes in light scattering that result from increases in molecular weight of the polymer. Increases in mass of 10% result in an easily measurable change in light scattering. It has been shown that the light scattering change is correlated with the incorporation of radioactive nucleotide, as measured by the filter binding method. Continuous monitoring of the time dependence of polymerization should prove useful in kinetic analysis of the polymerase reaction and related enzyme-DNA interactions.",

author = "Johnson, {K. A.} and Bryant, {F. R.} and Benkovic, {S. J.}",

note = "Funding Information: Supported by NIH Grants GM-26726 (KAJ) and GM-13306 (SJB).",

year = "1984",

month = jan,

doi = "10.1016/0003-2697(84)90324-5",

language = "English (US)",

volume = "136",

pages = "192--194",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Continuous assay for DNA polymerization by light scattering

AU - Johnson, K. A.

AU - Bryant, F. R.

AU - Benkovic, S. J.

N1 - Funding Information: Supported by NIH Grants GM-26726 (KAJ) and GM-13306 (SJB).

PY - 1984/1

Y1 - 1984/1

N2 - The time course of DNA polymerization, catalyzed by DNA polymerase I, can be monitored by following changes in light scattering that result from increases in molecular weight of the polymer. Increases in mass of 10% result in an easily measurable change in light scattering. It has been shown that the light scattering change is correlated with the incorporation of radioactive nucleotide, as measured by the filter binding method. Continuous monitoring of the time dependence of polymerization should prove useful in kinetic analysis of the polymerase reaction and related enzyme-DNA interactions.

AB - The time course of DNA polymerization, catalyzed by DNA polymerase I, can be monitored by following changes in light scattering that result from increases in molecular weight of the polymer. Increases in mass of 10% result in an easily measurable change in light scattering. It has been shown that the light scattering change is correlated with the incorporation of radioactive nucleotide, as measured by the filter binding method. Continuous monitoring of the time dependence of polymerization should prove useful in kinetic analysis of the polymerase reaction and related enzyme-DNA interactions.

UR - http://www.scopus.com/inward/record.url?scp=0021314371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021314371&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(84)90324-5

DO - 10.1016/0003-2697(84)90324-5

M3 - Article

C2 - 6711807

AN - SCOPUS:0021314371

SN - 0003-2697

VL - 136

SP - 192

EP - 194

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Continuous assay for DNA polymerization by light scattering

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this