Acetate-grown cultures of Methanosarcina thermophilla converted uniformly labeled [14C]Kepone to polar and nonpolar products with 86% of the Kepone degraded within the first 10 days. The titanium(III) citrate-reduced CO dehydrogenase enzyme complex isolated from M. thermophila also catalyzed the conversion of Kepone to polar and nonpolar products with a similar pattern as seen with whole cell cultures. Similar patterns of soluble Kepone decomposition products were obtained with reduced vitamin B12, reduced corrinoid cofactor (factor III) isolated from the CO dehydrogenase enzyme complex, and reduced coenzyme F430 isolated from the methyl coenzyme M methylreductase of M. thermophila.
All Science Journal Classification (ASJC) codes
- Molecular Biology