Conversion of Kepone by Methanosarcina thermophila

Peter E. Jablonski; Deborah J. Pheasant; James G. Ferry

doi:10.1016/0378-1097(96)00137-1

Conversion of Kepone by Methanosarcina thermophila

Peter E. Jablonski, Deborah J. Pheasant, James G. Ferry

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Acetate-grown cultures of Methanosarcina thermophilla converted uniformly labeled [¹⁴C]Kepone to polar and nonpolar products with 86% of the Kepone degraded within the first 10 days. The titanium(III) citrate-reduced CO dehydrogenase enzyme complex isolated from M. thermophila also catalyzed the conversion of Kepone to polar and nonpolar products with a similar pattern as seen with whole cell cultures. Similar patterns of soluble Kepone decomposition products were obtained with reduced vitamin B₁₂, reduced corrinoid cofactor (factor III) isolated from the CO dehydrogenase enzyme complex, and reduced coenzyme F₄₃₀ isolated from the methyl coenzyme M methylreductase of M. thermophila.

Original language	English (US)
Pages (from-to)	169-173
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	139
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1097(96)00137-1
State	Published - Jun 1 1996

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

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title = "Conversion of Kepone by Methanosarcina thermophila",

abstract = "Acetate-grown cultures of Methanosarcina thermophilla converted uniformly labeled [14C]Kepone to polar and nonpolar products with 86% of the Kepone degraded within the first 10 days. The titanium(III) citrate-reduced CO dehydrogenase enzyme complex isolated from M. thermophila also catalyzed the conversion of Kepone to polar and nonpolar products with a similar pattern as seen with whole cell cultures. Similar patterns of soluble Kepone decomposition products were obtained with reduced vitamin B12, reduced corrinoid cofactor (factor III) isolated from the CO dehydrogenase enzyme complex, and reduced coenzyme F430 isolated from the methyl coenzyme M methylreductase of M. thermophila.",

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AU - Jablonski, Peter E.

AU - Pheasant, Deborah J.

AU - Ferry, James G.

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N2 - Acetate-grown cultures of Methanosarcina thermophilla converted uniformly labeled [14C]Kepone to polar and nonpolar products with 86% of the Kepone degraded within the first 10 days. The titanium(III) citrate-reduced CO dehydrogenase enzyme complex isolated from M. thermophila also catalyzed the conversion of Kepone to polar and nonpolar products with a similar pattern as seen with whole cell cultures. Similar patterns of soluble Kepone decomposition products were obtained with reduced vitamin B12, reduced corrinoid cofactor (factor III) isolated from the CO dehydrogenase enzyme complex, and reduced coenzyme F430 isolated from the methyl coenzyme M methylreductase of M. thermophila.

AB - Acetate-grown cultures of Methanosarcina thermophilla converted uniformly labeled [14C]Kepone to polar and nonpolar products with 86% of the Kepone degraded within the first 10 days. The titanium(III) citrate-reduced CO dehydrogenase enzyme complex isolated from M. thermophila also catalyzed the conversion of Kepone to polar and nonpolar products with a similar pattern as seen with whole cell cultures. Similar patterns of soluble Kepone decomposition products were obtained with reduced vitamin B12, reduced corrinoid cofactor (factor III) isolated from the CO dehydrogenase enzyme complex, and reduced coenzyme F430 isolated from the methyl coenzyme M methylreductase of M. thermophila.

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Conversion of Kepone by Methanosarcina thermophila

Abstract

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