Correlation between crystalline cellulose structure and cellulose synthase complex shape: a spectroscopic study with unicellular freshwater alga Micrasterias

Shixin Huang; Sarah N. Kiemle; Mohamadamin Makarem; Seong H. Kim

doi:10.1007/s10570-019-02793-3

Correlation between crystalline cellulose structure and cellulose synthase complex shape: a spectroscopic study with unicellular freshwater alga Micrasterias

Shixin Huang, Sarah N. Kiemle, Mohamadamin Makarem, Seong H. Kim

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Abstract

Cellulose is synthesized into fibrils by enzyme complexes called cellulose synthase complexes (CSCs). We analyzed cellulose fibrils produced in a dividing unicellular freshwater alga Micrasterias with vibrational sum frequency generation (SFG) spectroscopy. It is known that cellulose in the daughter semi-cell of Micrasterias is synthesized by the isolated individual rosette-shape CSC, while cellulose in the mother semi-cell is produced by hexagonal arrays of rosette-shape CSCs. Comparing the SFG spectra of cellulose in these two regions of a single cell of Micrasterias showed intriguing correlations between the polymorphic structure and crystal size of cellulose and the shape and size of the CSCs. This information may provide a foundation needed to understand what determines the cellulose structure and crystallinity in plant cell walls.

Original language	English (US)
Pages (from-to)	57-69
Number of pages	13
Journal	Cellulose
Volume	27
Issue number	1
DOIs	https://doi.org/10.1007/s10570-019-02793-3
State	Published - Jan 1 2020

All Science Journal Classification (ASJC) codes

Polymers and Plastics

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10.1007/s10570-019-02793-3

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title = "Correlation between crystalline cellulose structure and cellulose synthase complex shape: a spectroscopic study with unicellular freshwater alga Micrasterias",

abstract = "Cellulose is synthesized into fibrils by enzyme complexes called cellulose synthase complexes (CSCs). We analyzed cellulose fibrils produced in a dividing unicellular freshwater alga Micrasterias with vibrational sum frequency generation (SFG) spectroscopy. It is known that cellulose in the daughter semi-cell of Micrasterias is synthesized by the isolated individual rosette-shape CSC, while cellulose in the mother semi-cell is produced by hexagonal arrays of rosette-shape CSCs. Comparing the SFG spectra of cellulose in these two regions of a single cell of Micrasterias showed intriguing correlations between the polymorphic structure and crystal size of cellulose and the shape and size of the CSCs. This information may provide a foundation needed to understand what determines the cellulose structure and crystallinity in plant cell walls.",

author = "Shixin Huang and Kiemle, {Sarah N.} and Mohamadamin Makarem and Kim, {Seong H.}",

note = "Funding Information: This work was supported by the Center for Lignocellulose Structure and Formation, Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences, under Award Number DE-SC0001090. The authors acknowledge David Domozych at Skidmore College for providing the Micrasterias culture for this study. The authors acknowledge Dr. Daniel Cosgrove for helpful discussion. Publisher Copyright: {\textcopyright} 2019, Springer Nature B.V.",

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doi = "10.1007/s10570-019-02793-3",

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AU - Makarem, Mohamadamin

AU - Kim, Seong H.

N1 - Funding Information: This work was supported by the Center for Lignocellulose Structure and Formation, Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences, under Award Number DE-SC0001090. The authors acknowledge David Domozych at Skidmore College for providing the Micrasterias culture for this study. The authors acknowledge Dr. Daniel Cosgrove for helpful discussion. Publisher Copyright: © 2019, Springer Nature B.V.

PY - 2020/1/1

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N2 - Cellulose is synthesized into fibrils by enzyme complexes called cellulose synthase complexes (CSCs). We analyzed cellulose fibrils produced in a dividing unicellular freshwater alga Micrasterias with vibrational sum frequency generation (SFG) spectroscopy. It is known that cellulose in the daughter semi-cell of Micrasterias is synthesized by the isolated individual rosette-shape CSC, while cellulose in the mother semi-cell is produced by hexagonal arrays of rosette-shape CSCs. Comparing the SFG spectra of cellulose in these two regions of a single cell of Micrasterias showed intriguing correlations between the polymorphic structure and crystal size of cellulose and the shape and size of the CSCs. This information may provide a foundation needed to understand what determines the cellulose structure and crystallinity in plant cell walls.

AB - Cellulose is synthesized into fibrils by enzyme complexes called cellulose synthase complexes (CSCs). We analyzed cellulose fibrils produced in a dividing unicellular freshwater alga Micrasterias with vibrational sum frequency generation (SFG) spectroscopy. It is known that cellulose in the daughter semi-cell of Micrasterias is synthesized by the isolated individual rosette-shape CSC, while cellulose in the mother semi-cell is produced by hexagonal arrays of rosette-shape CSCs. Comparing the SFG spectra of cellulose in these two regions of a single cell of Micrasterias showed intriguing correlations between the polymorphic structure and crystal size of cellulose and the shape and size of the CSCs. This information may provide a foundation needed to understand what determines the cellulose structure and crystallinity in plant cell walls.

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Correlation between crystalline cellulose structure and cellulose synthase complex shape: a spectroscopic study with unicellular freshwater alga Micrasterias

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