TY - JOUR
T1 - Correlation of cleavage of SNAP-25 with muscle function in a rat model of Botulinum neurotoxin type A induced paralysis
AU - Jurasinski, Christine V.
AU - Lieth, Erich
AU - Dang Do, An N.
AU - Schengrund, Cara Lynne
N1 - Funding Information:
The authors wish to thank Drs L.S. Jefferson and S.R. Kimball for use of the desk jet scanner, and Dr S. Levison for use of the Olympus BX-50 microscope and Paultek digital imaging equipment. This work was supported in part by Grant 5 F32 NS 10600-02 to C.V.J. and Grant NS35653 to C-L.S. from the National Institutes of Health.
PY - 2001
Y1 - 2001
N2 - Injection of botulinum neurotoxin serotype A (BoNT/A) into muscle results in cleavage of the synaptosomal associated protein of 25 kDa (SNAP-25) and relatively long-term paralysis. However, nerve-terminal sprouting, which appears to require intact SNAP-25, has been reported to occur much earlier. The difference between the long-term paralysis induced by injection of BoNT/A and the short time needed for sprouting led us to investigate the relationship between BoNT/A catalyzed cleavage of SNAP-25 and muscle function. The effect of BoNT/A on SNAP-25 present in nerve endings innervating gastrocnemius muscles of rats was monitored over time. Cleaved SNAP-25 was found in nerve terminals innervating the muscles within 24 h of inoculation with BoNT/A and was present more than 2 months later. Comparison of the ratios of cleaved to intact SNAP-25 from the onset of BoNT/A-induced paralysis until function was regained indicated that paralysis was probable when the ratio of cleaved to intact SNAP-25 was greater than 0.35.
AB - Injection of botulinum neurotoxin serotype A (BoNT/A) into muscle results in cleavage of the synaptosomal associated protein of 25 kDa (SNAP-25) and relatively long-term paralysis. However, nerve-terminal sprouting, which appears to require intact SNAP-25, has been reported to occur much earlier. The difference between the long-term paralysis induced by injection of BoNT/A and the short time needed for sprouting led us to investigate the relationship between BoNT/A catalyzed cleavage of SNAP-25 and muscle function. The effect of BoNT/A on SNAP-25 present in nerve endings innervating gastrocnemius muscles of rats was monitored over time. Cleaved SNAP-25 was found in nerve terminals innervating the muscles within 24 h of inoculation with BoNT/A and was present more than 2 months later. Comparison of the ratios of cleaved to intact SNAP-25 from the onset of BoNT/A-induced paralysis until function was regained indicated that paralysis was probable when the ratio of cleaved to intact SNAP-25 was greater than 0.35.
UR - https://www.scopus.com/pages/publications/0034990815
UR - https://www.scopus.com/inward/citedby.url?scp=0034990815&partnerID=8YFLogxK
U2 - 10.1016/S0041-0101(01)00082-4
DO - 10.1016/S0041-0101(01)00082-4
M3 - Article
C2 - 11384718
AN - SCOPUS:0034990815
SN - 0041-0101
VL - 39
SP - 1309
EP - 1315
JO - Toxicon
JF - Toxicon
IS - 9
ER -