TY - JOUR
T1 - Creation of cardiac tissue exhibiting mechanical integration of spheroids using 3D bioprinting
AU - Ong, Chin Siang
AU - Fukunishi, Takuma
AU - Nashed, Andrew
AU - Blazeski, Adriana
AU - Zhang, Huaitao
AU - Hardy, Samantha
AU - DiSilvestre, Deborah
AU - Vricella, Luca
AU - Conte, John
AU - Tung, Leslie
AU - Tomaselli, Gordon
AU - Hibino, Narutoshi
N1 - Publisher Copyright:
© 2017, Journal of Visualized Experiments. All rights reserved.
PY - 2017/7/2
Y1 - 2017/7/2
N2 - This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates. Within 3 days, beating spheroids form. These spheroids are then picked up by a nozzle using vacuum suction and assembled on a needle array using a 3D bioprinter. The spheroids are then allowed to fuse on the needle array. Three days after 3D bioprinting, the spheroids are removed as an intact patch, which is already spontaneously beating. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
AB - This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates. Within 3 days, beating spheroids form. These spheroids are then picked up by a nozzle using vacuum suction and assembled on a needle array using a 3D bioprinter. The spheroids are then allowed to fuse on the needle array. Three days after 3D bioprinting, the spheroids are removed as an intact patch, which is already spontaneously beating. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
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U2 - 10.3791/55438
DO - 10.3791/55438
M3 - Article
C2 - 28715377
AN - SCOPUS:85021742212
SN - 1940-087X
VL - 2017
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 125
M1 - e55438
ER -