TY - JOUR
T1 - CRISPR-based detection of SARS-CoV-2
T2 - A review from sample to result
AU - Nouri, Reza
AU - Tang, Zifan
AU - Dong, Ming
AU - Liu, Tianyi
AU - Kshirsagar, Aneesh
AU - Guan, Weihua
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/4/15
Y1 - 2021/4/15
N2 - The current pandemic of the 2019 novel coronavirus (COVID-19) caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2) has raised significant public health concern. Rapid, affordable, and accurate diagnostics of SARS-CoV-2 is essential for early treatment and control of the disease spread. In the past few years, CRISPR technology has shown great potential for highly sensitive and specific molecular diagnostics. Amid the ongoing COVID-19 pandemic, there is an increasing interest in implementing CRISPR-based diagnostic principles to develop fast and precise methods for detecting SARS-CoV-2. In this work, we reviewed and summarized these CRISPR-based diagnostic systems as well as their characteristics and challenges. We also provided future perspectives of CRISPR-based sensing towards point-of-care molecular diagnosis applications.
AB - The current pandemic of the 2019 novel coronavirus (COVID-19) caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2) has raised significant public health concern. Rapid, affordable, and accurate diagnostics of SARS-CoV-2 is essential for early treatment and control of the disease spread. In the past few years, CRISPR technology has shown great potential for highly sensitive and specific molecular diagnostics. Amid the ongoing COVID-19 pandemic, there is an increasing interest in implementing CRISPR-based diagnostic principles to develop fast and precise methods for detecting SARS-CoV-2. In this work, we reviewed and summarized these CRISPR-based diagnostic systems as well as their characteristics and challenges. We also provided future perspectives of CRISPR-based sensing towards point-of-care molecular diagnosis applications.
UR - http://www.scopus.com/inward/record.url?scp=85099657755&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85099657755&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2021.113012
DO - 10.1016/j.bios.2021.113012
M3 - Review article
C2 - 33497879
AN - SCOPUS:85099657755
SN - 0956-5663
VL - 178
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 113012
ER -