TY - JOUR
T1 - Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution
AU - Armache, Jean Paul
AU - Jarasch, Alexander
AU - Anger, Andreas M.
AU - Villa, Elizabeth
AU - Becker, Thomas
AU - Bhushan, Shashi
AU - Jossinet, Fabrice
AU - Habeck, Michael
AU - Dindar, Gülcin
AU - Franckenberg, Sibylle
AU - Marquez, Viter
AU - Mielke, Thorsten
AU - Thomm, Michael
AU - Berninghausen, Otto
AU - Beatrix, Birgitta
AU - Söding, Johannes
AU - Westhof, Eric
AU - Wilson, Daniel N.
AU - Beckmann, Roland
PY - 2010/11/16
Y1 - 2010/11/16
N2 - Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
AB - Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
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U2 - 10.1073/pnas.1009999107
DO - 10.1073/pnas.1009999107
M3 - Article
C2 - 20980660
AN - SCOPUS:78650546283
SN - 0027-8424
VL - 107
SP - 19748
EP - 19753
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 46
ER -