CryoEM reconstruction approaches to resolve asymmetric features

Daniel J. Goetschius, Hyunwook Lee, Susan Hafenstein

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations


Although icosahedral viruses have highly symmetrical capsid features, asymmetric structural elements are also present since the genome and minor structural proteins are usually incorporated without adhering to icosahedral symmetry. Besides this inherent asymmetry, interactions with the host during the virus life cycle are also asymmetric. However, until recently it was impossible to resolve high resolution asymmetric features during single-particle cryoEM image processing. This review summarizes the current approaches that can be used to visualize asymmetric structural features. We have included examples of advanced structural strategies developed to reveal unique features and asymmetry in icosahedral viruses.

Original languageEnglish (US)
Title of host publicationComplementary Strategies to Understand Virus Structure and Function
EditorsFélix A. Rey
PublisherAcademic Press Inc.
Number of pages19
ISBN (Print)9780128184561
StatePublished - 2019

Publication series

NameAdvances in Virus Research
ISSN (Print)0065-3527
ISSN (Electronic)1557-8399

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases


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