TY - JOUR
T1 - Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase
AU - Anandan, Anandhi
AU - Vallet, Corinne
AU - Coyle, Travis
AU - Moustafa, Ibrahim M.
AU - Vrielink, Alice
PY - 2010
Y1 - 2010
N2 - Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.
AB - Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.
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U2 - 10.1107/S1744309110017185
DO - 10.1107/S1744309110017185
M3 - Article
C2 - 20606279
AN - SCOPUS:77954442386
SN - 1744-3091
VL - 66
SP - 808
EP - 810
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 7
ER -