Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

Mark Staley; Lori C. Zeringue; Richard D. Kidd; B. Tracy Nixon; Gregory K. Farber

doi:10.1107/s0907444998004569

Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

Mark Staley, Lori C. Zeringue, Richard D. Kidd, B. Tracy Nixon, Gregory K. Farber

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 Å and exhibit the symmetry of space group I222 or I2₁2₁2₁. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 Å. The asymmetric unit contains a dimer and the crystals have a V_m of 2.16 Å³ Da^-1.

Original language	English (US)
Pages (from-to)	1416-1418
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	54
Issue number	6 PART II
DOIs	https://doi.org/10.1107/s0907444998004569
State	Published - 1998

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Structural Biology

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title = "Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain",

abstract = "The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 {\AA} and exhibit the symmetry of space group I222 or I212121. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 {\AA}. The asymmetric unit contains a dimer and the crystals have a Vm of 2.16 {\AA}3 Da-1.",

author = "Mark Staley and Zeringue, {Lori C.} and Kidd, {Richard D.} and Nixon, {B. Tracy} and Farber, {Gregory K.}",

year = "1998",

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language = "English (US)",

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issn = "0907-4449",

publisher = "John Wiley and Sons Inc.",

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AU - Staley, Mark

AU - Zeringue, Lori C.

AU - Kidd, Richard D.

AU - Nixon, B. Tracy

AU - Farber, Gregory K.

PY - 1998

Y1 - 1998

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JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 6 PART II

ER -

Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

Abstract

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