Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

Mark Staley; Lori C. Zeringue; Richard D. Kidd; B. Tracy Nixon; Gregory K. Farber

doi:10.1107/s0907444998004569

Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

Mark Staley, Lori C. Zeringue, Richard D. Kidd, B. Tracy Nixon, Gregory K. Farber

Plant Institute

Research output: Contribution to journal › Article › peer-review

Abstract

The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 Å and exhibit the symmetry of space group I222 or I2₁2₁2₁. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 Å. The asymmetric unit contains a dimer and the crystals have a V_m of 2.16 Å³ Da^-1.

Original language	English (US)
Pages (from-to)	1416-1418
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	54
Issue number	6 PART II
DOIs	https://doi.org/10.1107/s0907444998004569
State	Published - 1998

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Structural Biology

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title = "Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain",

abstract = "The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 {\AA} and exhibit the symmetry of space group I222 or I212121. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 {\AA}. The asymmetric unit contains a dimer and the crystals have a Vm of 2.16 {\AA}3 Da-1.",

author = "Mark Staley and Zeringue, \{Lori C.\} and Kidd, \{Richard D.\} and Nixon, \{B. Tracy\} and Farber, \{Gregory K.\}",

year = "1998",

doi = "10.1107/s0907444998004569",

language = "English (US)",

volume = "54",

pages = "1416--1418",

journal = "Acta Crystallographica Section D: Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

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T1 - Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

AU - Staley, Mark

AU - Zeringue, Lori C.

AU - Kidd, Richard D.

AU - Nixon, B. Tracy

AU - Farber, Gregory K.

PY - 1998

Y1 - 1998

N2 - The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 Å and exhibit the symmetry of space group I222 or I212121. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 Å. The asymmetric unit contains a dimer and the crystals have a Vm of 2.16 Å3 Da-1.

AB - The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 Å and exhibit the symmetry of space group I222 or I212121. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 Å. The asymmetric unit contains a dimer and the crystals have a Vm of 2.16 Å3 Da-1.

UR - https://www.scopus.com/pages/publications/0032211966

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SN - 0907-4449

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SP - 1416

EP - 1418

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 6 PART II

ER -

Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

Abstract

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