TY - JOUR
T1 - Crystallization of RNA/protein complexes
AU - Garber, Maria
AU - Gongadze, George
AU - Meshcheryakov, Vladimir
AU - Nikonov, Oleg
AU - Nikulin, Alexey
AU - Perederina, Anna
AU - Piendl, Wolfgang
AU - Serganov, Alexander
AU - Tishchenko, Svetlana
N1 - Funding Information:
I thank Thomas Steitz, Dieter Soll, Peter Moore, Joyce Sherman, and Alex Szewczak for useful discussions and permission to cite results prior to publication. The author was supported in part by NIH Grant GM-22778 to Thomas Steitz.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Different complexes of ribosomal proteins with specific rRNA fragments have been crystallized and studied by our group during the last six years. There are several factors important for successful crystallization of RNA/protein complexes, among them: length and content of RNA fragments, homogeneity of RNA and protein preparations, stability of the complexes, conditions for mixing RNA and protein components before crystallization, effect of Se-Met on RNA/protein complex crystal quality. In this paper we describe findings and methodical details, which helped us to succeed in obtaining X-ray quality crystals of several RNA/protein complexes.
AB - Different complexes of ribosomal proteins with specific rRNA fragments have been crystallized and studied by our group during the last six years. There are several factors important for successful crystallization of RNA/protein complexes, among them: length and content of RNA fragments, homogeneity of RNA and protein preparations, stability of the complexes, conditions for mixing RNA and protein components before crystallization, effect of Se-Met on RNA/protein complex crystal quality. In this paper we describe findings and methodical details, which helped us to succeed in obtaining X-ray quality crystals of several RNA/protein complexes.
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U2 - 10.1107/S0907444902014257
DO - 10.1107/S0907444902014257
M3 - Article
C2 - 12351883
AN - SCOPUS:0036795665
SN - 0907-4449
VL - 58
SP - 1664
EP - 1669
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 10 II
ER -