TY - JOUR
T1 - CSI1, PATROL1, and exocyst complex cooperate in delivery of cellulose synthase complexes to the plasma membrane
AU - Zhu, Xiaoyu
AU - Li, Shundai
AU - Pan, Songqin
AU - Xin, Xiaoran
AU - Gu, Ying
N1 - Funding Information:
ACKNOWLEDGMENTS. We thank Ryan Gutierrez and David Ehrhardt for providing the mCherry-TUA5 GV3101 agrobacteria strain; Herman Höfte for providing GFP–CESA3 and GFP–CESA6 transgenic seeds; Chris Somerville for providing YFP–CESA6 transgenic seeds; and Niko Geldner for providing mCherry-ARA7, -RabA1E, -RabA1G, -RabC1, -RabG3F, and -Rha1. Y.G. and X.Z. were supported by National Science Foundation Grant 1121375. S.L. and X.X. were supported by The Center for LignoCellulose Structure and Formation, an Energy Frontier Research Center funded by the Department of Energy, Office of Science, Basic Energy Sciences under Award DESC0001090.
Publisher Copyright:
© 2018 National Academy of Sciences. All Rights Reserved.
PY - 2018
Y1 - 2018
N2 - Cellulose synthesis occurs exclusively at the plasma membrane by cellulose synthase complexes (CSCs). Therefore, delivery of CSCs to discrete sites at the plasma membrane is critical for cellulose synthesis. Despite their significance, the delivery of CSCs is poorly understood. Here we used proteomics approaches, functional genetics, and live cell imaging to show that the de novo secretion of CSCs is mediated by cooperation among cellulose synthase interactive 1 (CSI1), the plant-specific protein PATROL1, and exocyst complex in Arabidopsis thaliana. We propose that CSI1 plays a role in marking the docking site, which allows CSCs-containing vesicles access to the plasma membrane through its interaction with microtubules. PATROL1 assists in exocytosis by its interaction with multiple components, including CSI1, CSCs, and exocyst subunits. Both PATROL1 and the exocyst complex determine the rate of delivery of CSCs to the plasma membrane. By monitoring the exocyst complex, PATROL1, CSI1, and CSCs dynamics in real time, we present a timeline of events for exocytosis of CSCs. Our findings provide unique insights into the evolution of exocytosis in eukaryotes.
AB - Cellulose synthesis occurs exclusively at the plasma membrane by cellulose synthase complexes (CSCs). Therefore, delivery of CSCs to discrete sites at the plasma membrane is critical for cellulose synthesis. Despite their significance, the delivery of CSCs is poorly understood. Here we used proteomics approaches, functional genetics, and live cell imaging to show that the de novo secretion of CSCs is mediated by cooperation among cellulose synthase interactive 1 (CSI1), the plant-specific protein PATROL1, and exocyst complex in Arabidopsis thaliana. We propose that CSI1 plays a role in marking the docking site, which allows CSCs-containing vesicles access to the plasma membrane through its interaction with microtubules. PATROL1 assists in exocytosis by its interaction with multiple components, including CSI1, CSCs, and exocyst subunits. Both PATROL1 and the exocyst complex determine the rate of delivery of CSCs to the plasma membrane. By monitoring the exocyst complex, PATROL1, CSI1, and CSCs dynamics in real time, we present a timeline of events for exocytosis of CSCs. Our findings provide unique insights into the evolution of exocytosis in eukaryotes.
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U2 - 10.1073/pnas.1800182115
DO - 10.1073/pnas.1800182115
M3 - Article
C2 - 29581258
AN - SCOPUS:85045118330
SN - 0027-8424
VL - 115
SP - E3578-E3587
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 15
ER -