TY - JOUR
T1 - Culture conditions affecting biodegradation components of the brown-rot fungus Gloeophyllum trabeum
AU - Varela, Elisa
AU - Mester, Tunde
AU - Tien, Ming
N1 - Funding Information:
Acknowledgements This work was supported in part by U.S. Department of Energy grant DE-FG02–87ER13690.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - To determine the liquid culture conditions under which the wood-degrading system of the brown-rot fungus Gloeophyllum trabeum is expressed, enzymes and metabolites from liquid and solid substrate cultures were characterized. Enzymes were analyzed by 2-D gel electrophoresis and also assayed. Growth conditions were varied by using liquid media containing: (1) low carbon, low nitrogen, (2) low carbon, high nitrogen, (3) high carbon, low nitrogen, or (4) high carbon, high nitrogen. The protein arrays expressed under the four conditions were very similar, and endo-1,4-β-glucanase (detected by 2-D gels) activity along with β-glucosidase, xylanase, and NADH/quinone oxidoreductase activities were detected. Maximal expression of the hydrolytic enzymes was observed in high carbon/high nitrogen medium, whereas the highest oxido-reductase activity was in the high carbon low nitrogen medium. Oxalate and 2,5-dimethoxybenzoquinone were detected under all culture conditions, with higher production in high carbon/low nitrogen medium. Cultures grown in this medium also yielded the highest rate of hydroxylation of p-hydroxybenzoic acid, yielding protocatechuic acid, a product of hydroxyl radical attack.
AB - To determine the liquid culture conditions under which the wood-degrading system of the brown-rot fungus Gloeophyllum trabeum is expressed, enzymes and metabolites from liquid and solid substrate cultures were characterized. Enzymes were analyzed by 2-D gel electrophoresis and also assayed. Growth conditions were varied by using liquid media containing: (1) low carbon, low nitrogen, (2) low carbon, high nitrogen, (3) high carbon, low nitrogen, or (4) high carbon, high nitrogen. The protein arrays expressed under the four conditions were very similar, and endo-1,4-β-glucanase (detected by 2-D gels) activity along with β-glucosidase, xylanase, and NADH/quinone oxidoreductase activities were detected. Maximal expression of the hydrolytic enzymes was observed in high carbon/high nitrogen medium, whereas the highest oxido-reductase activity was in the high carbon low nitrogen medium. Oxalate and 2,5-dimethoxybenzoquinone were detected under all culture conditions, with higher production in high carbon/low nitrogen medium. Cultures grown in this medium also yielded the highest rate of hydroxylation of p-hydroxybenzoic acid, yielding protocatechuic acid, a product of hydroxyl radical attack.
UR - http://www.scopus.com/inward/record.url?scp=0141991006&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141991006&partnerID=8YFLogxK
U2 - 10.1007/s00203-003-0583-y
DO - 10.1007/s00203-003-0583-y
M3 - Article
C2 - 12920506
AN - SCOPUS:0141991006
SN - 0302-8933
VL - 180
SP - 251
EP - 256
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 4
ER -