Cytochemical assay to localize respiratory activity in hairy root cultures

Usha Mukundan, Patrick T. Asplund, Wayne R. Curtis

Research output: Contribution to journalArticlepeer-review


When treated with nitroblue tetrazolium the hairy root cultures of Beta vulgaris (golden and red varieties), Brassica juncea, Cosmos sulphureus, Hyoscyamus muticus, Ocimum basilicum and Nicotiana tabaccum in Gamborg's liquid medium showed rapid and intense staining at the apical meristem and lateral root initials; this is indicative of elevated respiratory activity in these regions. The root hairs did not show any staining indicating their limited role in the overall respiratory budget of hairy roots grown as submerged cultures.

Original languageEnglish (US)
Pages (from-to)184-187
Number of pages4
JournalPhytomorphology: An International Journal of Plant Morphology
Issue number2
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Plant Science


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