TY - JOUR
T1 - Cytogenetic engineering in vivo
T2 - Restoration of biologic complement activity to C5 deficient mice by intravenous inoculation of hybrid cells
AU - Levy, N. L.
AU - Snyderman, R.
AU - Ladda, R. L.
AU - Lieberman, R.
PY - 1973
Y1 - 1973
N2 - Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5 deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5 Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic 'repair' in mammals.
AB - Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5 deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5 Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic 'repair' in mammals.
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U2 - 10.1073/pnas.70.11.3125
DO - 10.1073/pnas.70.11.3125
M3 - Article
C2 - 4131744
AN - SCOPUS:0015743227
SN - 0027-8424
VL - 70
SP - 3125
EP - 3129
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -