Deafness mutation in the MYO3A motor domain impairs actin protrusion elongation mechanism

Laura K. Gunther, Joseph A. Cirilo, Rohini Desetty, Christopher M. Yengo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Class III myosins are actin-based motors proposed to transport cargo to the distal tips of stereocilia in the inner ear hair cells and/or to participate in stereocilia length regulation, which is especially important during development. Mutations in the MYO3A gene are associated with delayed onset deafness. A previous study demonstrated that L697W, a dominant deafness mutation, disrupts MYO3A ATPase and motor properties but does not impair its ability to localize to the tips of actin protrusions. In the current study, we characterized the transient kinetic mechanism of the L697W motor ATPase cycle. Our kinetic analysis demonstrates that the mutation slows the ADP release and ATP hydrolysis steps, which results in a slight reduction in the duty ratio and slows detachment kinetics. Fluorescence recovery after photobleaching (FRAP) of filopodia tip localized L697W and WT MYO3A in COS-7 cells revealed that the mutant does not alter turnover or average intensity at the actin protrusion tips. We demonstrate that the mutation slows filopodia extension velocity in COS-7 cells which correlates with its twofold slower in vitro actin gliding velocity. Overall, this work allowed us to propose a model for how the motor properties of MYO3A are crucial for facilitating actin protrusion length regulation.

Original languageEnglish (US)
Article numberar5
JournalMolecular biology of the cell
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2022

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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