TY - JOUR
T1 - Decarboxylated S-adenosylmethionine in mammalian cells.
AU - Hibasami, H.
AU - Hoffman, J. L.
AU - Pegg, A. E.
N1 - Copyright:
Medline is the source for the citation and abstract of this record.
PY - 1980/7/25
Y1 - 1980/7/25
N2 - The content of decarboxylated S-adenosylmethionine in rat tissues was found to be of the order of 0.9 to 2.5 nmol/g wet weight, about 2 to 4% of the content of S-adenosylmethionine. Three methods were used for determinations: separation by high pressure liquid chromatography followed by quantitation using UV absorbance, separation by paper electrophoresis after labeling with radioactive methionine to get the ratio of S-adenosylmethionine to decarboxylated S-adenosylmethionine, and separation by electrophoresis followed by elution and assay by an isotope dilution technique using spermidine synthase. The three methods gave comparable results, although the labeling with methionine appeared to slightly overestimate levels in the liver. Hepatic decarboxylated S-adenosylmethionine content was reduced by more than 90% for at least 8 h by treatment with methylglyoxal bis(guanylhydrazone), a potent reversible inhibitor of S-adenosylmethionine decarboxylase, but had returned to control levels by 24 h. The related irreversible inhibitor, 1,1'-[(methylethanediylidene)-dinitrilo]bis(3-aminoguanidine), reduced levels slightly less to about 20% of control, but maintained them at this value for 2 days.
AB - The content of decarboxylated S-adenosylmethionine in rat tissues was found to be of the order of 0.9 to 2.5 nmol/g wet weight, about 2 to 4% of the content of S-adenosylmethionine. Three methods were used for determinations: separation by high pressure liquid chromatography followed by quantitation using UV absorbance, separation by paper electrophoresis after labeling with radioactive methionine to get the ratio of S-adenosylmethionine to decarboxylated S-adenosylmethionine, and separation by electrophoresis followed by elution and assay by an isotope dilution technique using spermidine synthase. The three methods gave comparable results, although the labeling with methionine appeared to slightly overestimate levels in the liver. Hepatic decarboxylated S-adenosylmethionine content was reduced by more than 90% for at least 8 h by treatment with methylglyoxal bis(guanylhydrazone), a potent reversible inhibitor of S-adenosylmethionine decarboxylase, but had returned to control levels by 24 h. The related irreversible inhibitor, 1,1'-[(methylethanediylidene)-dinitrilo]bis(3-aminoguanidine), reduced levels slightly less to about 20% of control, but maintained them at this value for 2 days.
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M3 - Article
C2 - 7391043
AN - SCOPUS:0019332751
SN - 0021-9258
VL - 255
SP - 6675
EP - 6678
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 14
ER -