Nisin spray treatments were tested for controlling Gram-positive bacteria attached to beef carcass surface tissue using a pilot scale model carcass washer. Section of lean and adipose tissues were inoculated with approximately 4 log10 cfu cm-2 of Brochothrix thermosphacta, Carnobacterium divergens or Listeria innocua. Following 28°C water or nisin sprays, bacterial populations were enumerated immediately and after incubation for 24 h at 4°C. Spray treatments with water did not significantly alter the bacterial populations at day 0 or 1 (< 1 log10 reduction). However, nisin spray treatments (5000 AU ml-1) reduced populations by 1·79 to 3·54 log10 cfu cm-2 at day 0 and by 1·97 to 3·60 log10 cfu cm-2 at day 1. This study demonstrates that spray washing is an effective means of applying bacteriocins and that these compounds may be useful as sanitizers of red meat carcasses.
All Science Journal Classification (ASJC) codes
- Food Science