TY - JOUR
T1 - Deep sequencing for discovery and evolutionary analysis of plant viruses
AU - Roossinck, Marilyn J.
PY - 2017/7/15
Y1 - 2017/7/15
N2 - The advent of next generation sequencing (NGS), or deep sequencing, has allowed great advances to be made in discovery, diagnostics, and evolutionary studies in plant viruses. Various methods have been used for enrichment for virus-specific nucleic acids, each of which have some drawbacks. Many novel viruses have been discovered in plants by NGS technologies, and there is a good deal of promise for more comprehensive studies in virus evolution. However, each aspect of using NGS has its caveats that need to be considered, and there is still a need for better tools of analysis, as well as method for validation of sequence variation.
AB - The advent of next generation sequencing (NGS), or deep sequencing, has allowed great advances to be made in discovery, diagnostics, and evolutionary studies in plant viruses. Various methods have been used for enrichment for virus-specific nucleic acids, each of which have some drawbacks. Many novel viruses have been discovered in plants by NGS technologies, and there is a good deal of promise for more comprehensive studies in virus evolution. However, each aspect of using NGS has its caveats that need to be considered, and there is still a need for better tools of analysis, as well as method for validation of sequence variation.
UR - http://www.scopus.com/inward/record.url?scp=85007543303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007543303&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2016.11.019
DO - 10.1016/j.virusres.2016.11.019
M3 - Review article
C2 - 27876625
AN - SCOPUS:85007543303
SN - 0168-1702
VL - 239
SP - 82
EP - 86
JO - Virus Research
JF - Virus Research
ER -
