Deep sequencing for discovery and evolutionary analysis of plant viruses

Marilyn J. Roossinck

Research output: Contribution to journalReview articlepeer-review

57 Scopus citations


The advent of next generation sequencing (NGS), or deep sequencing, has allowed great advances to be made in discovery, diagnostics, and evolutionary studies in plant viruses. Various methods have been used for enrichment for virus-specific nucleic acids, each of which have some drawbacks. Many novel viruses have been discovered in plants by NGS technologies, and there is a good deal of promise for more comprehensive studies in virus evolution. However, each aspect of using NGS has its caveats that need to be considered, and there is still a need for better tools of analysis, as well as method for validation of sequence variation.

Original languageEnglish (US)
Pages (from-to)82-86
Number of pages5
JournalVirus Research
StatePublished - Jul 15 2017

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Virology
  • Infectious Diseases


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