Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti

Cassandra Koh; M. Nurul Islam; Yixin H. Ye; Nunya Chotiwan; Barbara Graham; John T. Belisle; Konstantinos A. Kouremenos; Saravanan Dayalan; Dedreia L. Tull; Stephan Klatt; Rushika Perera; Elizabeth A. McGraw

doi:10.1038/s42003-020-01254-z

Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti

Cassandra Koh, M. Nurul Islam, Yixin H. Ye, Nunya Chotiwan, Barbara Graham, John T. Belisle, Konstantinos A. Kouremenos, Saravanan Dayalan, Dedreia L. Tull, Stephan Klatt, Rushika Perera, Elizabeth A. McGraw

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Competition between viruses and Wolbachia for host lipids is a proposed mechanism of Wolbachia-mediated virus blocking in insects. Yet, the metabolomic interaction between virus and symbiont within the mosquito has not been clearly defined. We compare the lipid profiles of Aedes aegypti mosquitoes bearing mono- or dual-infections of the Wolbachia wMel strain and dengue virus serotype 3 (DENV3). We found metabolic signatures of infection-induced intracellular events but little evidence to support direct competition between Wolbachia and virus for host lipids. Lipid profiles of dual-infected mosquitoes resemble those of DENV3 mono-infected mosquitoes, suggesting virus-driven modulation dominates over that of Wolbachia. Interestingly, knockdown of key metabolic enzymes suggests cardiolipins are host factors for DENV3 and Wolbachia replication. These findings define the Wolbachia-DENV3 metabolic interaction as indirectly antagonistic, rather than directly competitive, and reveal new research avenues with respect to mosquito × virus interactions at the molecular level.

Original language	English (US)
Article number	518
Journal	Communications Biology
Volume	3
Issue number	1
DOIs	https://doi.org/10.1038/s42003-020-01254-z
State	Published - Dec 1 2020

All Science Journal Classification (ASJC) codes

Medicine (miscellaneous)
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/s42003-020-01254-z

Cite this

@article{a162076542014e448fecf3d61be841f4,

title = "Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti",

abstract = "Competition between viruses and Wolbachia for host lipids is a proposed mechanism of Wolbachia-mediated virus blocking in insects. Yet, the metabolomic interaction between virus and symbiont within the mosquito has not been clearly defined. We compare the lipid profiles of Aedes aegypti mosquitoes bearing mono- or dual-infections of the Wolbachia wMel strain and dengue virus serotype 3 (DENV3). We found metabolic signatures of infection-induced intracellular events but little evidence to support direct competition between Wolbachia and virus for host lipids. Lipid profiles of dual-infected mosquitoes resemble those of DENV3 mono-infected mosquitoes, suggesting virus-driven modulation dominates over that of Wolbachia. Interestingly, knockdown of key metabolic enzymes suggests cardiolipins are host factors for DENV3 and Wolbachia replication. These findings define the Wolbachia-DENV3 metabolic interaction as indirectly antagonistic, rather than directly competitive, and reveal new research avenues with respect to mosquito × virus interactions at the molecular level.",

author = "Cassandra Koh and Islam, {M. Nurul} and Ye, {Yixin H.} and Nunya Chotiwan and Barbara Graham and Belisle, {John T.} and Kouremenos, {Konstantinos A.} and Saravanan Dayalan and Tull, {Dedreia L.} and Stephan Klatt and Rushika Perera and McGraw, {Elizabeth A.}",

note = "Publisher Copyright: {\textcopyright} 2020, The Author(s).",

year = "2020",

month = dec,

day = "1",

doi = "10.1038/s42003-020-01254-z",

language = "English (US)",

volume = "3",

journal = "Communications Biology",

issn = "2399-3642",

publisher = "Springer Nature",

number = "1",

}

TY - JOUR

T1 - Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti

AU - Koh, Cassandra

AU - Islam, M. Nurul

AU - Ye, Yixin H.

AU - Chotiwan, Nunya

AU - Graham, Barbara

AU - Belisle, John T.

AU - Kouremenos, Konstantinos A.

AU - Dayalan, Saravanan

AU - Tull, Dedreia L.

AU - Klatt, Stephan

AU - Perera, Rushika

AU - McGraw, Elizabeth A.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Competition between viruses and Wolbachia for host lipids is a proposed mechanism of Wolbachia-mediated virus blocking in insects. Yet, the metabolomic interaction between virus and symbiont within the mosquito has not been clearly defined. We compare the lipid profiles of Aedes aegypti mosquitoes bearing mono- or dual-infections of the Wolbachia wMel strain and dengue virus serotype 3 (DENV3). We found metabolic signatures of infection-induced intracellular events but little evidence to support direct competition between Wolbachia and virus for host lipids. Lipid profiles of dual-infected mosquitoes resemble those of DENV3 mono-infected mosquitoes, suggesting virus-driven modulation dominates over that of Wolbachia. Interestingly, knockdown of key metabolic enzymes suggests cardiolipins are host factors for DENV3 and Wolbachia replication. These findings define the Wolbachia-DENV3 metabolic interaction as indirectly antagonistic, rather than directly competitive, and reveal new research avenues with respect to mosquito × virus interactions at the molecular level.

AB - Competition between viruses and Wolbachia for host lipids is a proposed mechanism of Wolbachia-mediated virus blocking in insects. Yet, the metabolomic interaction between virus and symbiont within the mosquito has not been clearly defined. We compare the lipid profiles of Aedes aegypti mosquitoes bearing mono- or dual-infections of the Wolbachia wMel strain and dengue virus serotype 3 (DENV3). We found metabolic signatures of infection-induced intracellular events but little evidence to support direct competition between Wolbachia and virus for host lipids. Lipid profiles of dual-infected mosquitoes resemble those of DENV3 mono-infected mosquitoes, suggesting virus-driven modulation dominates over that of Wolbachia. Interestingly, knockdown of key metabolic enzymes suggests cardiolipins are host factors for DENV3 and Wolbachia replication. These findings define the Wolbachia-DENV3 metabolic interaction as indirectly antagonistic, rather than directly competitive, and reveal new research avenues with respect to mosquito × virus interactions at the molecular level.

UR - http://www.scopus.com/inward/record.url?scp=85091206062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85091206062&partnerID=8YFLogxK

U2 - 10.1038/s42003-020-01254-z

DO - 10.1038/s42003-020-01254-z

M3 - Article

C2 - 32948809

AN - SCOPUS:85091206062

SN - 2399-3642

VL - 3

JO - Communications Biology

JF - Communications Biology

IS - 1

M1 - 518

ER -

Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this