Detection and quantification of fluvalinate resistance in Varroa mites in Nebraska

P. A. Macedo, M. D. Ellis, B. D. Siegfried

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


In the fall of 1999, we surveyed honey bee colonies from 20 Nebraska counties for Varroa mites. We visited 75 apiaries and collected samples from 308 colonies. The colonies we sampled came from 17 beekeeping operations. Four of the operations (23.53%) had mite populations that were highly resistant to fluvalinate. Three (17.65%) had mite populations that were susceptible to fluvalinate, and 10 (58.82%) had mites that exhibited an intermediate resistance status. We selected one susceptible and one resistant colony from our survey to use to quantify the degree of resistance (resistance ratio). When we determined the LC50's for the susceptible and resistant populations, we found a resistance ratio of 12.95 for the fluvalinate-resistant mite population. Our results suggest that Nebraska beekeepers should check their colonies for fluvalinate-resistant mite populations before treating them with Apistan®.

Original languageEnglish (US)
Pages (from-to)523-526
Number of pages4
JournalAmerican Bee Journal
Issue number7
StatePublished - Jul 2002

All Science Journal Classification (ASJC) codes

  • Insect Science


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