TY - JOUR
T1 - Detection and quantitation of interleukin-2 from individual cells
AU - Viselli, Susan M.
AU - Mastro, Andrea M.
N1 - Funding Information:
This work was supported by PHS Grants CA24385, CA39891 and RCDA CA00705 to A.M.M. and by the NASA Center for Cell Research at Penn State. The authors thank Dr. W.C. Hymer for suggestions and helpful discussions. We also thank Sharon Pishak and Donna Johnson for preparation of bovine lymph nodes and Ellen Tullius for phlebotomy. We are grateful to Mary Karpinski and Rick Ball for their assistance with image analysis and to Trey White for his help with photography.
PY - 1989/12/20
Y1 - 1989/12/20
N2 - In this report we present the use of cell blotting for the detection of interleukin-2 (IL-2)-producing lymphocytes. This is a rapid and sensitive immunochemical method analogous to Western blotting of proteins. When combined with image analysis one can determine the percentages of IL-2 positive cells as well as quantitate the amount of IL-2 surrounding each cell. When bovine lymph node cells (bLNC) were stimulated with the combination of concanavalin A (ConA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) for 24 h, 46.4 ± 0.6% stained positive for IL-2 and, on average, each cell produced 0.92 ± 0.6 pg of IL-2 in 24 h. Phytohemagglutinin (PHA) and TPA-stimulated human peripheral blood mononuclear cells (hPBMC) produced approximately the same amount, 0.86 ± 0.4 pg of IL-2 per cell in 24 h; 45.6 ± 3.6% stained positive for IL-2.
AB - In this report we present the use of cell blotting for the detection of interleukin-2 (IL-2)-producing lymphocytes. This is a rapid and sensitive immunochemical method analogous to Western blotting of proteins. When combined with image analysis one can determine the percentages of IL-2 positive cells as well as quantitate the amount of IL-2 surrounding each cell. When bovine lymph node cells (bLNC) were stimulated with the combination of concanavalin A (ConA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) for 24 h, 46.4 ± 0.6% stained positive for IL-2 and, on average, each cell produced 0.92 ± 0.6 pg of IL-2 in 24 h. Phytohemagglutinin (PHA) and TPA-stimulated human peripheral blood mononuclear cells (hPBMC) produced approximately the same amount, 0.86 ± 0.4 pg of IL-2 per cell in 24 h; 45.6 ± 3.6% stained positive for IL-2.
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U2 - 10.1016/0022-1759(89)90084-7
DO - 10.1016/0022-1759(89)90084-7
M3 - Article
C2 - 2607145
AN - SCOPUS:0024829272
SN - 0022-1759
VL - 125
SP - 115
EP - 124
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -