Detection and quantitation of interleukin-2 from individual cells

Susan M. Viselli; Andrea M. Mastro

doi:10.1016/0022-1759(89)90084-7

Detection and quantitation of interleukin-2 from individual cells

Susan M. Viselli, Andrea M. Mastro

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In this report we present the use of cell blotting for the detection of interleukin-2 (IL-2)-producing lymphocytes. This is a rapid and sensitive immunochemical method analogous to Western blotting of proteins. When combined with image analysis one can determine the percentages of IL-2 positive cells as well as quantitate the amount of IL-2 surrounding each cell. When bovine lymph node cells (bLNC) were stimulated with the combination of concanavalin A (ConA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) for 24 h, 46.4 ± 0.6% stained positive for IL-2 and, on average, each cell produced 0.92 ± 0.6 pg of IL-2 in 24 h. Phytohemagglutinin (PHA) and TPA-stimulated human peripheral blood mononuclear cells (hPBMC) produced approximately the same amount, 0.86 ± 0.4 pg of IL-2 per cell in 24 h; 45.6 ± 3.6% stained positive for IL-2.

Original language	English (US)
Pages (from-to)	115-124
Number of pages	10
Journal	Journal of Immunological Methods
Volume	125
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(89)90084-7
State	Published - Dec 20 1989

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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10.1016/0022-1759(89)90084-7

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@article{48399ab835414b55be0b05bd76b4e758,

title = "Detection and quantitation of interleukin-2 from individual cells",

abstract = "In this report we present the use of cell blotting for the detection of interleukin-2 (IL-2)-producing lymphocytes. This is a rapid and sensitive immunochemical method analogous to Western blotting of proteins. When combined with image analysis one can determine the percentages of IL-2 positive cells as well as quantitate the amount of IL-2 surrounding each cell. When bovine lymph node cells (bLNC) were stimulated with the combination of concanavalin A (ConA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) for 24 h, 46.4 ± 0.6\% stained positive for IL-2 and, on average, each cell produced 0.92 ± 0.6 pg of IL-2 in 24 h. Phytohemagglutinin (PHA) and TPA-stimulated human peripheral blood mononuclear cells (hPBMC) produced approximately the same amount, 0.86 ± 0.4 pg of IL-2 per cell in 24 h; 45.6 ± 3.6\% stained positive for IL-2.",

author = "Viselli, \{Susan M.\} and Mastro, \{Andrea M.\}",

note = "Funding Information: This work was supported by PHS Grants CA24385, CA39891 and RCDA CA00705 to A.M.M. and by the NASA Center for Cell Research at Penn State. The authors thank Dr. W.C. Hymer for suggestions and helpful discussions. We also thank Sharon Pishak and Donna Johnson for preparation of bovine lymph nodes and Ellen Tullius for phlebotomy. We are grateful to Mary Karpinski and Rick Ball for their assistance with image analysis and to Trey White for his help with photography.",

year = "1989",

month = dec,

day = "20",

doi = "10.1016/0022-1759(89)90084-7",

language = "English (US)",

volume = "125",

pages = "115--124",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

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T1 - Detection and quantitation of interleukin-2 from individual cells

AU - Viselli, Susan M.

AU - Mastro, Andrea M.

N1 - Funding Information: This work was supported by PHS Grants CA24385, CA39891 and RCDA CA00705 to A.M.M. and by the NASA Center for Cell Research at Penn State. The authors thank Dr. W.C. Hymer for suggestions and helpful discussions. We also thank Sharon Pishak and Donna Johnson for preparation of bovine lymph nodes and Ellen Tullius for phlebotomy. We are grateful to Mary Karpinski and Rick Ball for their assistance with image analysis and to Trey White for his help with photography.

PY - 1989/12/20

Y1 - 1989/12/20

N2 - In this report we present the use of cell blotting for the detection of interleukin-2 (IL-2)-producing lymphocytes. This is a rapid and sensitive immunochemical method analogous to Western blotting of proteins. When combined with image analysis one can determine the percentages of IL-2 positive cells as well as quantitate the amount of IL-2 surrounding each cell. When bovine lymph node cells (bLNC) were stimulated with the combination of concanavalin A (ConA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) for 24 h, 46.4 ± 0.6% stained positive for IL-2 and, on average, each cell produced 0.92 ± 0.6 pg of IL-2 in 24 h. Phytohemagglutinin (PHA) and TPA-stimulated human peripheral blood mononuclear cells (hPBMC) produced approximately the same amount, 0.86 ± 0.4 pg of IL-2 per cell in 24 h; 45.6 ± 3.6% stained positive for IL-2.

AB - In this report we present the use of cell blotting for the detection of interleukin-2 (IL-2)-producing lymphocytes. This is a rapid and sensitive immunochemical method analogous to Western blotting of proteins. When combined with image analysis one can determine the percentages of IL-2 positive cells as well as quantitate the amount of IL-2 surrounding each cell. When bovine lymph node cells (bLNC) were stimulated with the combination of concanavalin A (ConA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) for 24 h, 46.4 ± 0.6% stained positive for IL-2 and, on average, each cell produced 0.92 ± 0.6 pg of IL-2 in 24 h. Phytohemagglutinin (PHA) and TPA-stimulated human peripheral blood mononuclear cells (hPBMC) produced approximately the same amount, 0.86 ± 0.4 pg of IL-2 per cell in 24 h; 45.6 ± 3.6% stained positive for IL-2.

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JO - Journal of Immunological Methods

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ER -

Detection and quantitation of interleukin-2 from individual cells

Abstract

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