Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay

B. R. Gulati; S. Munir; D. P. Patnayak; S. M. Goyal; V. Kapur

doi:10.1128/JCM.39.8.2967-2970.2001

Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay

B. R. Gulati
, S. Munir
, D. P. Patnayak
, S. M. Goyal
, V. Kapur

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

Original language	English (US)
Pages (from-to)	2967-2970
Number of pages	4
Journal	Journal of clinical microbiology
Volume	39
Issue number	8
DOIs	https://doi.org/10.1128/JCM.39.8.2967-2970.2001
State	Published - 2001

All Science Journal Classification (ASJC) codes

Microbiology (medical)

Access to Document

10.1128/JCM.39.8.2967-2970.2001

Cite this

@article{2a59b958780b45f5a26990f6814c863c,

title = "Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay",

abstract = "The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.",

author = "Gulati, \{B. R.\} and S. Munir and Patnayak, \{D. P.\} and Goyal, \{S. M.\} and V. Kapur",

year = "2001",

doi = "10.1128/JCM.39.8.2967-2970.2001",

language = "English (US)",

volume = "39",

pages = "2967--2970",

journal = "Journal of clinical microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "8",

}

TY - JOUR

T1 - Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay

AU - Gulati, B. R.

AU - Munir, S.

AU - Patnayak, D. P.

AU - Goyal, S. M.

AU - Kapur, V.

PY - 2001

Y1 - 2001

N2 - The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

AB - The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

UR - https://www.scopus.com/pages/publications/0034884541

UR - https://www.scopus.com/pages/publications/0034884541#tab=citedBy

U2 - 10.1128/JCM.39.8.2967-2970.2001

DO - 10.1128/JCM.39.8.2967-2970.2001

M3 - Article

C2 - 11474024

AN - SCOPUS:0034884541

SN - 0095-1137

VL - 39

SP - 2967

EP - 2970

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 8

ER -

Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this