TY - GEN
T1 - Detection of biotin-streptavidin interaction using RF interdigitated capacitive cavity
AU - Porwal, Pratik
AU - Syed, Azeemuddin
AU - Bhimalapuram, Prabhakar
AU - Sau, Tapan Kumar
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2017/6/5
Y1 - 2017/6/5
N2 - In this paper, biotin streptavidin interaction has been sensed at microwave frequencies with the help of designed sensor. It takes advantage of two pole filter topology where interdigitated capacitor (IDC) is used as a coupling zone between resonators for higher sensitivity. Due to permittivity change of biotin streptavidin combination, shift in resonant frequency arise whereas due to conductivity, magnitude of reflection coefficient changes. The frequency range of 10 GHz-25 GHz (γ-dispersion region) is used as it is effective for label free analysis of biotin streptavidin. Due to homogeneous E-field between the fingers of IDC, placement position of biotin-streptavidin will not affect the sensitivity. Significant frequency shifts as high as 19 MHz, 45 MHz and 70 MHz are observed for concentrations as low as 10 ng/ml, 20 ng/ml and 30 ng/ml of biotin-streptavidin respectively. Simulation results suggest that proposed biosensor is sensitive even for concentration of 5 ng/ml of sample.
AB - In this paper, biotin streptavidin interaction has been sensed at microwave frequencies with the help of designed sensor. It takes advantage of two pole filter topology where interdigitated capacitor (IDC) is used as a coupling zone between resonators for higher sensitivity. Due to permittivity change of biotin streptavidin combination, shift in resonant frequency arise whereas due to conductivity, magnitude of reflection coefficient changes. The frequency range of 10 GHz-25 GHz (γ-dispersion region) is used as it is effective for label free analysis of biotin streptavidin. Due to homogeneous E-field between the fingers of IDC, placement position of biotin-streptavidin will not affect the sensitivity. Significant frequency shifts as high as 19 MHz, 45 MHz and 70 MHz are observed for concentrations as low as 10 ng/ml, 20 ng/ml and 30 ng/ml of biotin-streptavidin respectively. Simulation results suggest that proposed biosensor is sensitive even for concentration of 5 ng/ml of sample.
UR - https://www.scopus.com/pages/publications/85021392292
UR - https://www.scopus.com/pages/publications/85021392292#tab=citedBy
U2 - 10.1109/IMaRC.2016.7939624
DO - 10.1109/IMaRC.2016.7939624
M3 - Conference contribution
AN - SCOPUS:85021392292
T3 - 2016 IEEE MTTS International Microwave and RF Conference, IMaRC 2016 - Proceedings
BT - 2016 IEEE MTTS International Microwave and RF Conference, IMaRC 2016 - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2016 IEEE MTTS International Microwave and RF Conference, IMaRC 2016
Y2 - 5 December 2016 through 9 December 2016
ER -