TY - JOUR
T1 - Detection of insertion/deletion polymorphisms from challenged samples using the Investigator DIPplex® Kit
AU - Klein, Rebecca
AU - Neumann, Cedric
AU - Roy, Reena
N1 - Publisher Copyright:
© 2014 Elsevier Ireland Ltd. All rights reserved.
PY - 2015/5
Y1 - 2015/5
N2 - This research focuses on detection of bi-allelic insertion/deletion polymorphisms (InDels) from challenged samples using the Investigator DIPplex® Kit from Qiagen. The study included analyzing body fluids from humans, as well as pristine and degraded samples. For the purpose of assessing species specificity, samples from various animals were included. At first, an analytical threshold (AT) for the detection of alleles was established based on an assessment of the noise in the system. Then, InDel profiles were obtained from samples exposed to detrimental environmental conditions, washed bloodstains, lipsticks, ChapStick®, ancient Croatian bone samples, and every day products such as toothbrushes and dental floss. Concordant profiles were obtained from different body fluids of the same donor. InDel profiles were also generated successfully when body fluids were deposited on substrates and directly amplified without pre-treatment with buffer or washing reagents. InDels can provide additional information when only partial STR profiles are generated from challenged samples.
AB - This research focuses on detection of bi-allelic insertion/deletion polymorphisms (InDels) from challenged samples using the Investigator DIPplex® Kit from Qiagen. The study included analyzing body fluids from humans, as well as pristine and degraded samples. For the purpose of assessing species specificity, samples from various animals were included. At first, an analytical threshold (AT) for the detection of alleles was established based on an assessment of the noise in the system. Then, InDel profiles were obtained from samples exposed to detrimental environmental conditions, washed bloodstains, lipsticks, ChapStick®, ancient Croatian bone samples, and every day products such as toothbrushes and dental floss. Concordant profiles were obtained from different body fluids of the same donor. InDel profiles were also generated successfully when body fluids were deposited on substrates and directly amplified without pre-treatment with buffer or washing reagents. InDels can provide additional information when only partial STR profiles are generated from challenged samples.
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U2 - 10.1016/j.fsigen.2014.11.020
DO - 10.1016/j.fsigen.2014.11.020
M3 - Article
C2 - 25528661
AN - SCOPUS:84919470283
SN - 1872-4973
VL - 16
SP - 29
EP - 37
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
ER -