TY - JOUR
T1 - Detection of Leishmania Antigen in Kala Azar Patients Using Monoclonal Antibodies
AU - Sinha, Raghu
AU - Arora, Sunil
AU - Datta, Usha
AU - Sehgal, Shobha
PY - 1992
Y1 - 1992
N2 - Twenty-one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani. Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42–116 kDa were selected as ‘capture antibodies’ and compared with specific anti-leishmania antisera for detection of circulating leishmania antigens in kala azar patients’ sera in a competitive-enzyme-linked immunosorbent assay system (ELISA). The anti-leishmania antisera could detect circulating antigen in 30% of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4%. The efficacy of Hyb.6 was how-ever low (31.7%). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.
AB - Twenty-one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani. Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42–116 kDa were selected as ‘capture antibodies’ and compared with specific anti-leishmania antisera for detection of circulating leishmania antigens in kala azar patients’ sera in a competitive-enzyme-linked immunosorbent assay system (ELISA). The anti-leishmania antisera could detect circulating antigen in 30% of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4%. The efficacy of Hyb.6 was how-ever low (31.7%). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.
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U2 - 10.1111/j.1348-0421.1992.tb02038.x
DO - 10.1111/j.1348-0421.1992.tb02038.x
M3 - Article
C2 - 1406366
AN - SCOPUS:0026684852
SN - 0385-5600
VL - 36
SP - 391
EP - 400
JO - Microbiology and Immunology
JF - Microbiology and Immunology
IS - 4
ER -