Detection of Leishmania Antigen in Kala Azar Patients Using Monoclonal Antibodies

Raghu Sinha, Sunil Arora, Usha Datta, Shobha Sehgal

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Twenty-one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani. Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42–116 kDa were selected as ‘capture antibodies’ and compared with specific anti-leishmania antisera for detection of circulating leishmania antigens in kala azar patients’ sera in a competitive-enzyme-linked immunosorbent assay system (ELISA). The anti-leishmania antisera could detect circulating antigen in 30% of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4%. The efficacy of Hyb.6 was how-ever low (31.7%). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.

Original languageEnglish (US)
Pages (from-to)391-400
Number of pages10
JournalMicrobiology and Immunology
Issue number4
StatePublished - 1992

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology


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