Gas chromatography-negative ion chemical ionization-mass spectrometry-selected ion monitoring (GC-NICI-MS-SIM) was employed to detect tetramethyl ether derivatives of tetraols released upon hydrolysis of DNA and globin adducts derived from diol epoxides of polynuclear aromatic hydrocarbons (PAH). The methodology involves the following steps: 1) isolation of DNA or globin; 2) mild acid hydrolysis under vacuum; 3) isolation of the resulting tetraols and derivatization to the corresponding tetramethyl ethers using CH3I; 4) HPLC purification followed by GC-NICI-MS-SIM analysis. Analysis of human globin samples by this method indicates the presence of adducts which release chrysene-1,2,3,4-tetraol and benzo[a]pyrene-7,8,9,10-tetraol in smokers and nonsmokers. The level of BP-diol epoxide globin adducts in smokers was significantly higher than that in nonsmokers (2.75 vs 0.96 fmol/mg globin). The concentration of chrysene diol-epoxide globin adducts in the same subjects was about two orders of magnitude greater than that of BP-diol epoxide adducts. BP-diol epoxide DNA adducts have also been identified in surgical specimens of human lung tissue.
All Science Journal Classification (ASJC) codes
- Polymers and Plastics
- Organic Chemistry
- Materials Chemistry