Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus

Stacey L. Lance; Cris Hagen; Travis C. Glenn; Nicole A. Freidenfelds; Tracy Langkilde

doi:10.1007/s12686-009-9057-0

Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus

Stacey L. Lance
, Cris Hagen
, Travis C. Glenn
, Nicole A. Freidenfelds
, Tracy Langkilde

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We isolated and characterized 17 microsatellite loci from the eastern fence lizard, Sceloporus undulatus. Loci were screened in 24 individuals of S. undulatus. The number of alleles per locus ranged from 8 to 22, observed heterozygosity ranged from 0.174 to 0.913, and the probability of identity values ranged from 0.008 to 0.1. These new loci provide tools for examining the population genetics of eastern fence lizards and for assessing the evolutionary impacts the invasive red imported fire ant has on the lizard.

Original language	English (US)
Pages (from-to)	233-236
Number of pages	4
Journal	Conservation Genetics Resources
Volume	1
Issue number	1
DOIs	https://doi.org/10.1007/s12686-009-9057-0
State	Published - Dec 2009

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-009-9057-0

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@article{d7724459044c422da97bd39eb4593330,

title = "Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus",

abstract = "We isolated and characterized 17 microsatellite loci from the eastern fence lizard, Sceloporus undulatus. Loci were screened in 24 individuals of S. undulatus. The number of alleles per locus ranged from 8 to 22, observed heterozygosity ranged from 0.174 to 0.913, and the probability of identity values ranged from 0.008 to 0.1. These new loci provide tools for examining the population genetics of eastern fence lizards and for assessing the evolutionary impacts the invasive red imported fire ant has on the lizard.",

author = "Lance, \{Stacey L.\} and Cris Hagen and Glenn, \{Travis C.\} and Freidenfelds, \{Nicole A.\} and Tracy Langkilde",

note = "Funding Information: Acknowledgements This work was supported by funds from Pennsylvania State University Department of Biology. Permits were provided by the Alabama Department of Conservation and Natural Resources (\# 3568, \#4059, \#4453), Arkansas Game and Fish Commission (\# 031520061, \#030220075, \#022520081), Mississippi Department of Wildlife, Fisheries and Parks (to T. Langkilde), US Fish and Wildlife Service (\# 43620-NOX-2006-02), and USDA Forest Service (\# 2710 and SO-FW-FY06-08). The Institutional Animal Care and Use Committee at Yale University (\#2006-11020) and Penn State University (\#27696) approved this research. Tissue collection was assisted by Nisha Ligon and Katherine Boronow. We would also like to thank Tara Fulton for lab guidance. Manuscript preparation was partially supported by the Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.",

year = "2009",

month = dec,

doi = "10.1007/s12686-009-9057-0",

language = "English (US)",

volume = "1",

pages = "233--236",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

publisher = "Springer Science and Business Media B.V.",

number = "1",

}

TY - JOUR

T1 - Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus

AU - Lance, Stacey L.

AU - Hagen, Cris

AU - Glenn, Travis C.

AU - Freidenfelds, Nicole A.

AU - Langkilde, Tracy

N1 - Funding Information: Acknowledgements This work was supported by funds from Pennsylvania State University Department of Biology. Permits were provided by the Alabama Department of Conservation and Natural Resources (# 3568, #4059, #4453), Arkansas Game and Fish Commission (# 031520061, #030220075, #022520081), Mississippi Department of Wildlife, Fisheries and Parks (to T. Langkilde), US Fish and Wildlife Service (# 43620-NOX-2006-02), and USDA Forest Service (# 2710 and SO-FW-FY06-08). The Institutional Animal Care and Use Committee at Yale University (#2006-11020) and Penn State University (#27696) approved this research. Tissue collection was assisted by Nisha Ligon and Katherine Boronow. We would also like to thank Tara Fulton for lab guidance. Manuscript preparation was partially supported by the Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.

PY - 2009/12

Y1 - 2009/12

N2 - We isolated and characterized 17 microsatellite loci from the eastern fence lizard, Sceloporus undulatus. Loci were screened in 24 individuals of S. undulatus. The number of alleles per locus ranged from 8 to 22, observed heterozygosity ranged from 0.174 to 0.913, and the probability of identity values ranged from 0.008 to 0.1. These new loci provide tools for examining the population genetics of eastern fence lizards and for assessing the evolutionary impacts the invasive red imported fire ant has on the lizard.

AB - We isolated and characterized 17 microsatellite loci from the eastern fence lizard, Sceloporus undulatus. Loci were screened in 24 individuals of S. undulatus. The number of alleles per locus ranged from 8 to 22, observed heterozygosity ranged from 0.174 to 0.913, and the probability of identity values ranged from 0.008 to 0.1. These new loci provide tools for examining the population genetics of eastern fence lizards and for assessing the evolutionary impacts the invasive red imported fire ant has on the lizard.

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UR - https://www.scopus.com/pages/publications/84859992213#tab=citedBy

U2 - 10.1007/s12686-009-9057-0

DO - 10.1007/s12686-009-9057-0

M3 - Article

AN - SCOPUS:84859992213

SN - 1877-7252

VL - 1

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EP - 236

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 1

ER -

Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus

Abstract

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