Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry

John T. Bernert; Wayman E. Turner; James L. Pirkle; Connie S. Sosnoff; James R. Akins; Mary K. Waldrep; Qinghong Ann; Thomas R. Covey; Wanda E. Whitfield; Elaine W. Gunter; Barbara B. Miller; Donald G. Patterson; Larry L. Needham; W. Harry Hannon; Eric J. Sampson

doi:10.1093/clinchem/43.12.2281

Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry

John T. Bernert, Wayman E. Turner, James L. Pirkle, Connie S. Sosnoff, James R. Akins, Mary K. Waldrep, Qinghong Ann, Thomas R. Covey, Wanda E. Whitfield, Elaine W. Gunter, Barbara B. Miller, Donald G. Patterson, Larry L. Needham, W. Harry Hannon, Eric J. Sampson

Forensic Science Program

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316 Scopus citations

Abstract

We describe a sensitive and specific method for measuring cotinine in serum by HPLC coupled to an atmospheric pressure chemical ionization tandem mass spectrometer. This method can analyze 100 samples/day on a routine basis, and its limit of detection of 50 ng/L makes it applicable to the analysis of samples from nonsmokers potentially exposed to environmental tobacco smoke. Analytical accuracy has been demonstrated from the analysis of NIST cotinine standards and from comparative analyses by both the current method and gas chromatography/high-resolution mass spectrometry. Precision has been examined through the repetitive analysis of a series of bench and blind QC materials. This method has been applied to the analysis of cotinine in serum samples collected as part of the Third National Health and Nutrition Examination Survey (NHANES III).

Original language	English (US)
Pages (from-to)	2281-2291
Number of pages	11
Journal	Clinical chemistry
Volume	43
Issue number	12
DOIs	https://doi.org/10.1093/clinchem/43.12.2281
State	Published - 1997

All Science Journal Classification (ASJC) codes

General Medicine

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10.1093/clinchem/43.12.2281

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Bernert, J. T., Turner, W. E., Pirkle, J. L., Sosnoff, C. S., Akins, J. R., Waldrep, M. K., Ann, Q., Covey, T. R., Whitfield, W. E., Gunter, E. W., Miller, B. B., Patterson, D. G., Needham, L. L., Hannon, W. H., & Sampson, E. J. (1997). Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry. Clinical chemistry, 43(12), 2281-2291. https://doi.org/10.1093/clinchem/43.12.2281

@article{616595360b284829beb91d1347a4de0a,

title = "Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry",

abstract = "We describe a sensitive and specific method for measuring cotinine in serum by HPLC coupled to an atmospheric pressure chemical ionization tandem mass spectrometer. This method can analyze 100 samples/day on a routine basis, and its limit of detection of 50 ng/L makes it applicable to the analysis of samples from nonsmokers potentially exposed to environmental tobacco smoke. Analytical accuracy has been demonstrated from the analysis of NIST cotinine standards and from comparative analyses by both the current method and gas chromatography/high-resolution mass spectrometry. Precision has been examined through the repetitive analysis of a series of bench and blind QC materials. This method has been applied to the analysis of cotinine in serum samples collected as part of the Third National Health and Nutrition Examination Survey (NHANES III).",

author = "Bernert, {John T.} and Turner, {Wayman E.} and Pirkle, {James L.} and Sosnoff, {Connie S.} and Akins, {James R.} and Waldrep, {Mary K.} and Qinghong Ann and Covey, {Thomas R.} and Whitfield, {Wanda E.} and Gunter, {Elaine W.} and Miller, {Barbara B.} and Patterson, {Donald G.} and Needham, {Larry L.} and Hannon, {W. Harry} and Sampson, {Eric J.}",

year = "1997",

doi = "10.1093/clinchem/43.12.2281",

language = "English (US)",

volume = "43",

pages = "2281--2291",

journal = "Clinical chemistry",

issn = "0009-9147",

publisher = "Oxford University Press",

number = "12",

}

Bernert, JT, Turner, WE, Pirkle, JL, Sosnoff, CS, Akins, JR, Waldrep, MK, Ann, Q, Covey, TR, Whitfield, WE, Gunter, EW, Miller, BB, Patterson, DG, Needham, LL, Hannon, WH & Sampson, EJ 1997, 'Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry', Clinical chemistry, vol. 43, no. 12, pp. 2281-2291. https://doi.org/10.1093/clinchem/43.12.2281

Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry. / Bernert, John T.; Turner, Wayman E.; Pirkle, James L. et al.
In: Clinical chemistry, Vol. 43, No. 12, 1997, p. 2281-2291.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry

AU - Bernert, John T.

AU - Turner, Wayman E.

AU - Pirkle, James L.

AU - Sosnoff, Connie S.

AU - Akins, James R.

AU - Waldrep, Mary K.

AU - Ann, Qinghong

AU - Covey, Thomas R.

AU - Whitfield, Wanda E.

AU - Gunter, Elaine W.

AU - Miller, Barbara B.

AU - Patterson, Donald G.

AU - Needham, Larry L.

AU - Hannon, W. Harry

AU - Sampson, Eric J.

PY - 1997

Y1 - 1997

N2 - We describe a sensitive and specific method for measuring cotinine in serum by HPLC coupled to an atmospheric pressure chemical ionization tandem mass spectrometer. This method can analyze 100 samples/day on a routine basis, and its limit of detection of 50 ng/L makes it applicable to the analysis of samples from nonsmokers potentially exposed to environmental tobacco smoke. Analytical accuracy has been demonstrated from the analysis of NIST cotinine standards and from comparative analyses by both the current method and gas chromatography/high-resolution mass spectrometry. Precision has been examined through the repetitive analysis of a series of bench and blind QC materials. This method has been applied to the analysis of cotinine in serum samples collected as part of the Third National Health and Nutrition Examination Survey (NHANES III).

AB - We describe a sensitive and specific method for measuring cotinine in serum by HPLC coupled to an atmospheric pressure chemical ionization tandem mass spectrometer. This method can analyze 100 samples/day on a routine basis, and its limit of detection of 50 ng/L makes it applicable to the analysis of samples from nonsmokers potentially exposed to environmental tobacco smoke. Analytical accuracy has been demonstrated from the analysis of NIST cotinine standards and from comparative analyses by both the current method and gas chromatography/high-resolution mass spectrometry. Precision has been examined through the repetitive analysis of a series of bench and blind QC materials. This method has been applied to the analysis of cotinine in serum samples collected as part of the Third National Health and Nutrition Examination Survey (NHANES III).

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SN - 0009-9147

VL - 43

SP - 2281

EP - 2291

JO - Clinical chemistry

JF - Clinical chemistry

IS - 12

ER -

Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry

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