Abstract
Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F ehromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.
Original language | English (US) |
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Pages (from-to) | 1016-1022 |
Number of pages | 7 |
Journal | Journal of food protection |
Volume | 73 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2010 |
All Science Journal Classification (ASJC) codes
- Food Science
- Microbiology