Development of an improved protocol for the isolation and detection of enterobacter sakazakii (Cronobacter) from powdered infant formula

Yi Chen; Kwang Yong Song; Eric W. Brown; Keith A. Lampel

doi:10.4315/0362-028X-73.6.1016

Development of an improved protocol for the isolation and detection of enterobacter sakazakii (Cronobacter) from powdered infant formula

Yi Chen, Kwang Yong Song, Eric W. Brown, Keith A. Lampel

Food Science

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F ehromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.

Original language	English (US)
Pages (from-to)	1016-1022
Number of pages	7
Journal	Journal of food protection
Volume	73
Issue number	6
DOIs	https://doi.org/10.4315/0362-028X-73.6.1016
State	Published - Jun 2010

All Science Journal Classification (ASJC) codes

Food Science
Microbiology

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10.4315/0362-028X-73.6.1016

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title = "Development of an improved protocol for the isolation and detection of enterobacter sakazakii (Cronobacter) from powdered infant formula",

abstract = "Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F ehromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.",

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AU - Brown, Eric W.

AU - Lampel, Keith A.

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AB - Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F ehromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.

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Development of an improved protocol for the isolation and detection of enterobacter sakazakii (Cronobacter) from powdered infant formula

Abstract

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