TY - JOUR
T1 - Development of an Organic Lateral Resolution Test Device for Imaging Mass Spectrometry
AU - Passarelli, Melissa K.
AU - Wang, Jun
AU - Mohammadi, Amir Saeid
AU - Trouillon, Raphael
AU - Gilmore, Ian
AU - Ewing, Andrew G.
N1 - Publisher Copyright:
© 2014 American Chemical Society.
PY - 2014/10/7
Y1 - 2014/10/7
N2 - An organic lateral resolution test device has been developed to measure the performance of imaging mass spectrometry (IMS) systems. The device contains periodic gratings of polyethylene glycol (PEG) and lipid bars covering a wide range of spatial frequencies. Microfabrication technologies were employed to produce well-defined chemical interfaces, which allow lateral resolution to be assessed using the edge-spread function (ESF). In addition, the design of the device allows for the direct measurement of the modulation transfer function (MTF) to assess image quality. Scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) were used to characterize the device. TOF-SIMS imaging was used to measure the chemical displacement of biomolecules in matrix-assisted laser desorption/ionization (MALDI) matrix crystals. In a proof-of-concept experiment, the platform was also used to evaluate MALDI matrix application methods, specifically aerosol spray and sublimation methods.
AB - An organic lateral resolution test device has been developed to measure the performance of imaging mass spectrometry (IMS) systems. The device contains periodic gratings of polyethylene glycol (PEG) and lipid bars covering a wide range of spatial frequencies. Microfabrication technologies were employed to produce well-defined chemical interfaces, which allow lateral resolution to be assessed using the edge-spread function (ESF). In addition, the design of the device allows for the direct measurement of the modulation transfer function (MTF) to assess image quality. Scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) were used to characterize the device. TOF-SIMS imaging was used to measure the chemical displacement of biomolecules in matrix-assisted laser desorption/ionization (MALDI) matrix crystals. In a proof-of-concept experiment, the platform was also used to evaluate MALDI matrix application methods, specifically aerosol spray and sublimation methods.
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U2 - 10.1021/ac501228x
DO - 10.1021/ac501228x
M3 - Article
C2 - 25137365
AN - SCOPUS:84907896232
SN - 0003-2700
VL - 86
SP - 9473
EP - 9480
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -