TY - JOUR
T1 - Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using illumina sequencing
AU - Wu, Yawen
AU - Zhang, Ruhua
AU - Staton, Margaret
AU - Schlarbaum, Scott E.
AU - Coggeshall, Mark V.
AU - Romero-Severson, Jeanne
AU - Carlson, John E.
AU - Zembower, Nicole
AU - Liang, Haiying
AU - Xu, Yi
AU - Drautz-Moses, Daniela I.
AU - Schuster, Stephan C.
AU - Gailing, Oliver
N1 - Funding Information:
The study was funded by the National Science Foundation Plant Genome Research Program (# TRPGRA2 IOS-1025974). Additional support was made available by the Ecosystem Science Center and the Biotech Research 348 Center of Michigan Technological University. Ruhua Zhang was funded by a scholarship from Linyi University, China. We would like to thank Majid Ghafarian for help with the lab work.
Publisher Copyright:
© 2017, Editura Silvica. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Twenty new polymorphic genic SSRs (EST-SSRs) and 13 genomic SSRs were developed in honeylocust (Gleditsia triacanthos) using Illumina transcriptome and low-coverage genome sequencing. A diversity panel of 40 honeylocust samples covering large parts of the species distribution range was characterized. As expected the level of genetic variation was lower in EST-SSRs than for non-genic genomic SSRs. This is the first report of EST-SSRs for honeylocust. All markers are polymorphic and produce clear single locus amplification products and can be used for genetic diversity and gene flow analyses. The transcriptome sequencing data provide a rich resource for new marker development.
AB - Twenty new polymorphic genic SSRs (EST-SSRs) and 13 genomic SSRs were developed in honeylocust (Gleditsia triacanthos) using Illumina transcriptome and low-coverage genome sequencing. A diversity panel of 40 honeylocust samples covering large parts of the species distribution range was characterized. As expected the level of genetic variation was lower in EST-SSRs than for non-genic genomic SSRs. This is the first report of EST-SSRs for honeylocust. All markers are polymorphic and produce clear single locus amplification products and can be used for genetic diversity and gene flow analyses. The transcriptome sequencing data provide a rich resource for new marker development.
UR - https://www.scopus.com/pages/publications/85038349147
UR - https://www.scopus.com/pages/publications/85038349147#tab=citedBy
U2 - 10.15287/afr.2017.819
DO - 10.15287/afr.2017.819
M3 - Article
AN - SCOPUS:85038349147
SN - 1844-8135
VL - 60
SP - 343
EP - 350
JO - Annals of Forest Research
JF - Annals of Forest Research
IS - 2
ER -