TY - JOUR
T1 - Development of microsatellite markers from four hawaiian corals
T2 - Acropora cytherea, Fungia scutaria, Montipora capitata and Porites lobata
AU - Concepcion, Gregory T.
AU - Polato, Nicholas R.
AU - Baums, Iliana B.
AU - Toonen, Robert J.
N1 - Funding Information:
Acknowledgments We’d like to thank R. Brainard, M. Crepeau, E. Franklin, S. Godwin, M. Iacchei, J. Maragos, J. Salerno, D. Skillings, M. Stat, M. Timers, the staff of the Papaha¯naumokua¯kea Marine National Monument, and the crew of the R/V Hi’ialakai for sample collection, field and laboratory assistance. We also thank members of the Toonen-Bowen laboratory for their discussion, advice and support, and the NSF-EPSCoR Evolutionary Genetics facility at HIMB. This research was funded in part by the National Science Foundation (Grant OCE-0550294 to IBB, OCE-0623678 to RJT, an NSF pre-doctoral fellowship to NRP, and an NSF EPSCoR pre-doctoral fellowship to GTC) and the National Oceanic and Atmospheric Administration (NMSP MOA#2005-008/66882). This is contribution 1366 from the Hawai‘i Institute of Marine Biology and SOEST 7818.
PY - 2010/12
Y1 - 2010/12
N2 - Comprehensive population genetic studies of coral communities are comparatively rare, because of the scarcity of population genetic markers. The Hawaiian archipelago offers a unique perspective into understanding the population genetic structuring of this ecologically important group of organisms. Here we report the development of microsatellite marker libraries from holobiont extracts of four corals: Acropora cytherea (n = 50), Fungia scutaria (n = 118), Montipora capitata (n = 140) and Porites lobata (n = 149). Blast searches indicate that these libraries contain microsatellites from both the coral host and Symbiodinium endosymbionts from each coral. In addition, we also present redesigned primers for the nuclear coding region (atpsb) for use in M. capitata. We report testing and optimization for seven of these microsatellites from A. cytherea, and eight microsatellites and the atpsb locus from M. capitata. Using 25 individuals per species collected from each French Frigate Shoals (FF) and Johnston Atoll (JO), the number of alleles per locus ranged from 2 to 9. Expected heterozygosities ranged from 0.38 to 0.85 and 0.08 to 0.87 for A. cytherea and M. capitata, respectively. We expect that these libraries will be a valuable resource and provide additional useful microsatellite markers for both the coral host and zooxanthellae.
AB - Comprehensive population genetic studies of coral communities are comparatively rare, because of the scarcity of population genetic markers. The Hawaiian archipelago offers a unique perspective into understanding the population genetic structuring of this ecologically important group of organisms. Here we report the development of microsatellite marker libraries from holobiont extracts of four corals: Acropora cytherea (n = 50), Fungia scutaria (n = 118), Montipora capitata (n = 140) and Porites lobata (n = 149). Blast searches indicate that these libraries contain microsatellites from both the coral host and Symbiodinium endosymbionts from each coral. In addition, we also present redesigned primers for the nuclear coding region (atpsb) for use in M. capitata. We report testing and optimization for seven of these microsatellites from A. cytherea, and eight microsatellites and the atpsb locus from M. capitata. Using 25 individuals per species collected from each French Frigate Shoals (FF) and Johnston Atoll (JO), the number of alleles per locus ranged from 2 to 9. Expected heterozygosities ranged from 0.38 to 0.85 and 0.08 to 0.87 for A. cytherea and M. capitata, respectively. We expect that these libraries will be a valuable resource and provide additional useful microsatellite markers for both the coral host and zooxanthellae.
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U2 - 10.1007/s12686-009-9118-4
DO - 10.1007/s12686-009-9118-4
M3 - Article
AN - SCOPUS:79960930201
SN - 1877-7252
VL - 2
SP - 11
EP - 15
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 1
ER -