TY - GEN
T1 - Development of polymeric micro/nanostructures for gene delivery
AU - Alyounes, Dania
AU - Yengo, Christopher
AU - Doran, Tim
AU - Lu, Qi
AU - Gonsalves, Kenneth
PY - 2007/1/1
Y1 - 2007/1/1
N2 - Pluronic® polymers were screened for binding affinity to oligoribonucleotides (ORN), also termed OLIGOS, using fluorescence techniques. F127 (Mw 12600 g/mole) proved to have the strongest binding affinity. Urethane linkages were introduced into F127 and two other Pluronics, F38 (Mw 4700 glmole) and F77 (Mw 6600 g/mole), to prepare polymers that were multiples of their respective initial molecular weights. A series of these polyurethane Pluronics were screened for binding affinity. Fluorescence studies showed a relationship between the molecular weight of the P0 units and the binding affinity in the structures of the newly synthesized poly Pluronics especially in the case of F77. Light scattering confirmed the binding affinity between the oligo and F77 polymers. Particle size analysis showed an exponential increase until the critical micelle concentration. Other analogs of these polymers also studied for their binding affinity were a poly(amino ester) and protective, interactive, noncondensing polymers (PINC).
AB - Pluronic® polymers were screened for binding affinity to oligoribonucleotides (ORN), also termed OLIGOS, using fluorescence techniques. F127 (Mw 12600 g/mole) proved to have the strongest binding affinity. Urethane linkages were introduced into F127 and two other Pluronics, F38 (Mw 4700 glmole) and F77 (Mw 6600 g/mole), to prepare polymers that were multiples of their respective initial molecular weights. A series of these polyurethane Pluronics were screened for binding affinity. Fluorescence studies showed a relationship between the molecular weight of the P0 units and the binding affinity in the structures of the newly synthesized poly Pluronics especially in the case of F77. Light scattering confirmed the binding affinity between the oligo and F77 polymers. Particle size analysis showed an exponential increase until the critical micelle concentration. Other analogs of these polymers also studied for their binding affinity were a poly(amino ester) and protective, interactive, noncondensing polymers (PINC).
UR - http://www.scopus.com/inward/record.url?scp=70349908258&partnerID=8YFLogxK
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U2 - 10.1557/proc-1019-ff05-13
DO - 10.1557/proc-1019-ff05-13
M3 - Conference contribution
AN - SCOPUS:70349908258
SN - 9781605604268
T3 - Materials Research Society Symposium Proceedings
SP - 41
EP - 48
BT - Materials Research Society Symposium Proceedings - Engineered Nanoscale Materials for the Diagnosis and Treatment of Disease
PB - Materials Research Society
T2 - Engineered Nanoscale Materials for the Diagnosis and Treatment of Disease - 2007 MRS Spring Meeting
Y2 - 9 April 2007 through 13 April 2007
ER -