Development of tri- and tetra- nucleotide polysomic microsatellite markers for characterization of American ginseng (Panax quinquefolius L.) genetic diversity and population structuring

John A. Young; Michael S. Eackles; Marcus J. Springmann; Tim L. King

doi:10.1007/s12686-012-9653-2

Development of tri- and tetra- nucleotide polysomic microsatellite markers for characterization of American ginseng (Panax quinquefolius L.) genetic diversity and population structuring

John A. Young, Michael S. Eackles, Marcus J. Springmann, Tim L. King

Ecosystem Science and Management

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6 Scopus citations

Abstract

American ginseng (Panax quinquefolius L., syn. Panax quinquefolium) is an herbaceous perennial plant native to eastern North America that is both harvested from the wild and cultivated in farm and woodlot settings to supply the international market. We describe the development of microsatellite markers to better characterize Panax quinquefolius population structure and phylogeography. We developed and tested 12 microsattelite loci, scored as 106 psuedo-dominant alleles due to the tetraploid nature of P. quinquefolius. We employed the newly developed markers to characterize population structure of field sampled populations from 13 states in comparison with commercially cultivated samples. Results reveal distinct phylogeographic and population structuring as well as a high degree of selfing in this CITES protected species.

Original language	English (US)
Pages (from-to)	833-836
Number of pages	4
Journal	Conservation Genetics Resources
Volume	4
Issue number	4
DOIs	https://doi.org/10.1007/s12686-012-9653-2
State	Published - Dec 2012

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-012-9653-2

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title = "Development of tri- and tetra- nucleotide polysomic microsatellite markers for characterization of American ginseng (Panax quinquefolius L.) genetic diversity and population structuring",

abstract = "American ginseng (Panax quinquefolius L., syn. Panax quinquefolium) is an herbaceous perennial plant native to eastern North America that is both harvested from the wild and cultivated in farm and woodlot settings to supply the international market. We describe the development of microsatellite markers to better characterize Panax quinquefolius population structure and phylogeography. We developed and tested 12 microsattelite loci, scored as 106 psuedo-dominant alleles due to the tetraploid nature of P. quinquefolius. We employed the newly developed markers to characterize population structure of field sampled populations from 13 states in comparison with commercially cultivated samples. Results reveal distinct phylogeographic and population structuring as well as a high degree of selfing in this CITES protected species.",

author = "Young, {John A.} and Eackles, {Michael S.} and Springmann, {Marcus J.} and King, {Tim L.}",

note = "Funding Information: Acknowledgments This project was funded by the USGS-USFWS Science Support Partnership. We thank Patricia Ford of USFWS for initiating this project and we thank Cheryl Morrison of USGS for review of this paper. We also thank Bob Beyfuss and Ken Mudge of Cornell University Ag. Extension for access to fresh root material. Reference to trade names does not imply endorsement by the U.S. Government.",

year = "2012",

month = dec,

doi = "10.1007/s12686-012-9653-2",

language = "English (US)",

volume = "4",

pages = "833--836",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

publisher = "Springer Science and Business Media B.V.",

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Development of tri- and tetra- nucleotide polysomic microsatellite markers for characterization of American ginseng (Panax quinquefolius L.) genetic diversity and population structuring. / Young, John A.; Eackles, Michael S.; Springmann, Marcus J. et al.
In: Conservation Genetics Resources, Vol. 4, No. 4, 12.2012, p. 833-836.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development of tri- and tetra- nucleotide polysomic microsatellite markers for characterization of American ginseng (Panax quinquefolius L.) genetic diversity and population structuring

AU - Young, John A.

AU - Eackles, Michael S.

AU - Springmann, Marcus J.

AU - King, Tim L.

N1 - Funding Information: Acknowledgments This project was funded by the USGS-USFWS Science Support Partnership. We thank Patricia Ford of USFWS for initiating this project and we thank Cheryl Morrison of USGS for review of this paper. We also thank Bob Beyfuss and Ken Mudge of Cornell University Ag. Extension for access to fresh root material. Reference to trade names does not imply endorsement by the U.S. Government.

PY - 2012/12

Y1 - 2012/12

N2 - American ginseng (Panax quinquefolius L., syn. Panax quinquefolium) is an herbaceous perennial plant native to eastern North America that is both harvested from the wild and cultivated in farm and woodlot settings to supply the international market. We describe the development of microsatellite markers to better characterize Panax quinquefolius population structure and phylogeography. We developed and tested 12 microsattelite loci, scored as 106 psuedo-dominant alleles due to the tetraploid nature of P. quinquefolius. We employed the newly developed markers to characterize population structure of field sampled populations from 13 states in comparison with commercially cultivated samples. Results reveal distinct phylogeographic and population structuring as well as a high degree of selfing in this CITES protected species.

AB - American ginseng (Panax quinquefolius L., syn. Panax quinquefolium) is an herbaceous perennial plant native to eastern North America that is both harvested from the wild and cultivated in farm and woodlot settings to supply the international market. We describe the development of microsatellite markers to better characterize Panax quinquefolius population structure and phylogeography. We developed and tested 12 microsattelite loci, scored as 106 psuedo-dominant alleles due to the tetraploid nature of P. quinquefolius. We employed the newly developed markers to characterize population structure of field sampled populations from 13 states in comparison with commercially cultivated samples. Results reveal distinct phylogeographic and population structuring as well as a high degree of selfing in this CITES protected species.

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Development of tri- and tetra- nucleotide polysomic microsatellite markers for characterization of American ginseng (Panax quinquefolius L.) genetic diversity and population structuring

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