TY - CHAP
T1 - Diet in vitamin a research
AU - Ross, A. Catharine
PY - 2010
Y1 - 2010
N2 - A properly formulated diet is an essential underpinning for all in vivo research. This chapter focuses on the use of diet in retinoid research from two perspectives: human research, in which diet is usually variable and analysis of dietary intake is paramount to interpreting the study's results, and animal (rodent) research, in which diet is imposed as a factor in the experimental design, and the diet consumed is usually monotonous. Many standard rodent diets are nonpurified and the amount of vitamin A in the diet is unknown. Moreover, it is likely to be much higher than expected from the label. By using a well-formulated purified diet with an exact amount of vitamin A, retinoid status in rodents can be closely controlled to create specific physiological conditions that represent the wide range of vitamin A status present in human populations.
AB - A properly formulated diet is an essential underpinning for all in vivo research. This chapter focuses on the use of diet in retinoid research from two perspectives: human research, in which diet is usually variable and analysis of dietary intake is paramount to interpreting the study's results, and animal (rodent) research, in which diet is imposed as a factor in the experimental design, and the diet consumed is usually monotonous. Many standard rodent diets are nonpurified and the amount of vitamin A in the diet is unknown. Moreover, it is likely to be much higher than expected from the label. By using a well-formulated purified diet with an exact amount of vitamin A, retinoid status in rodents can be closely controlled to create specific physiological conditions that represent the wide range of vitamin A status present in human populations.
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U2 - 10.1007/978-1-60327-325-1_17
DO - 10.1007/978-1-60327-325-1_17
M3 - Chapter
C2 - 20552436
AN - SCOPUS:77956419274
SN - 9781603273244
T3 - Methods in Molecular Biology
SP - 295
EP - 313
BT - Retinoids
ER -