TY - JOUR
T1 - Dietary conjugated linoleic acid normalizes impaired glucose tolerance in the Zucker diabetic fatty fa/fa rat
AU - Houseknecht, Karen L.
AU - Heuvel, John P.Vanden
AU - Moya-Camarena, Silvia Y.
AU - Portocarrero, Carla P.
AU - Peck, Louise W.
AU - Nickel, Kwangok P.
AU - Belury, Martha A.
N1 - Funding Information:
The authors thank K.-L. Liu, C. Kavanaugh, E. Snyder, J. Siegel-Willot and M. Leininger for their expert technical assistance, and Dr. Juleen Zierath for critically reading the manuscript. Authors thank LifeScan (Milpitas, CA) for donating a portion of the glucose analysis strips used in this study. CLA was generously donated by Pharmanutrients (Lake Bluff, IL). Troglitazone (Rezulin) was provided in part by Parke Davis (Ann Arbor, MI). This work was supported by a grant from the National Cattleman's Beef Association [J.P.V.H., L.W.P., and M.A.B.] and grants from the Purdue Agricultural Research Programs [K.L.H, M.A.B.]. S. Moya-Camarena was supported by a scholarship from CONACYT (Mexico). Purdue University ARP Manuscript 15582.
PY - 1998/3/27
Y1 - 1998/3/27
N2 - Conjugated linoleic acid (CLA) is a naturally occurring fatty acid which has anti-carcinogenic and anti-atherogenic properties. CLA activates PPARα in liver, and shares functional similarities to ligands of PPARγ, the thiazolidinediones, which are potent insulin sensitizers. We provide the first evidence that CLA is able to normalize impaired glucose tolerance and improve hyperinsulinemia in the pre-diabetic ZDF rat. Additionally, dietary CLA increased steady state levels of aP2 mRNA in adipose tissue of fatty ZDF rats compared to controls, consistent with activation of PPARγ. The insulin sensitizing effects of CLA are due, at least in part, to activation of PPARγ since increasing levels of CLA induced a dose-dependent transactivation of PPARγ in CV-1 cells cotransfected with PPARγ and PPRE X 3-luciferase reporter construct. CLA effects on glucose tolerance and glucose homeostasis indicate that dietary CLA may prove to be an important therapy for the prevention and treatment of NIDDM.
AB - Conjugated linoleic acid (CLA) is a naturally occurring fatty acid which has anti-carcinogenic and anti-atherogenic properties. CLA activates PPARα in liver, and shares functional similarities to ligands of PPARγ, the thiazolidinediones, which are potent insulin sensitizers. We provide the first evidence that CLA is able to normalize impaired glucose tolerance and improve hyperinsulinemia in the pre-diabetic ZDF rat. Additionally, dietary CLA increased steady state levels of aP2 mRNA in adipose tissue of fatty ZDF rats compared to controls, consistent with activation of PPARγ. The insulin sensitizing effects of CLA are due, at least in part, to activation of PPARγ since increasing levels of CLA induced a dose-dependent transactivation of PPARγ in CV-1 cells cotransfected with PPARγ and PPRE X 3-luciferase reporter construct. CLA effects on glucose tolerance and glucose homeostasis indicate that dietary CLA may prove to be an important therapy for the prevention and treatment of NIDDM.
UR - http://www.scopus.com/inward/record.url?scp=0032571089&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032571089&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.8303
DO - 10.1006/bbrc.1998.8303
M3 - Article
C2 - 9535724
AN - SCOPUS:0032571089
SN - 0006-291X
VL - 244
SP - 678
EP - 682
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -