Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip

Ahmad Ahsan Nawaz, Ruth Helmus Nissly, Peng Li, Yasir M. Shariff, Lin Wang, Tony Jun Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Here we present an integrated, "microfluidic drifting" based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. Our device is capable of analyzing human blood for cell counting and diagnosis of diseases at a throughput of ∼3,754 events/sec. 10 μm Flow-Check Fluorospheres polystyrene (PS) calibration beads (Beckman Coulter, Inc.) were used for characterization of Forward Scatter, Side Scatter, and Fluorescent signals revealing coefficient of variation (CV) of 3.92%, 3.82%, and 9.81%, respectively. Comparison of Immonological analysis of human blood from healthy donor via microfluidic drifting flow cytometry chip revealed its comparable performance to commercial flow cytometer. Furthermore, to demonstrate the credibility of our device, CD4+ lymphocyte cell count of 18.4% in comparison to 19.2% from commercial flow cytometer suggests that this device possesses the potential of a useful POC diagnostic tool.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1068-1070
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/TerritoryUnited States
CitySan Antonio
Period10/26/1410/30/14

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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