TY - GEN
T1 - Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip
AU - Nawaz, Ahmad Ahsan
AU - Nissly, Ruth Helmus
AU - Li, Peng
AU - Shariff, Yasir M.
AU - Wang, Lin
AU - Huang, Tony Jun
N1 - Publisher Copyright:
© 14CBMS.
PY - 2014
Y1 - 2014
N2 - Here we present an integrated, "microfluidic drifting" based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. Our device is capable of analyzing human blood for cell counting and diagnosis of diseases at a throughput of ∼3,754 events/sec. 10 μm Flow-Check Fluorospheres polystyrene (PS) calibration beads (Beckman Coulter, Inc.) were used for characterization of Forward Scatter, Side Scatter, and Fluorescent signals revealing coefficient of variation (CV) of 3.92%, 3.82%, and 9.81%, respectively. Comparison of Immonological analysis of human blood from healthy donor via microfluidic drifting flow cytometry chip revealed its comparable performance to commercial flow cytometer. Furthermore, to demonstrate the credibility of our device, CD4+ lymphocyte cell count of 18.4% in comparison to 19.2% from commercial flow cytometer suggests that this device possesses the potential of a useful POC diagnostic tool.
AB - Here we present an integrated, "microfluidic drifting" based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. Our device is capable of analyzing human blood for cell counting and diagnosis of diseases at a throughput of ∼3,754 events/sec. 10 μm Flow-Check Fluorospheres polystyrene (PS) calibration beads (Beckman Coulter, Inc.) were used for characterization of Forward Scatter, Side Scatter, and Fluorescent signals revealing coefficient of variation (CV) of 3.92%, 3.82%, and 9.81%, respectively. Comparison of Immonological analysis of human blood from healthy donor via microfluidic drifting flow cytometry chip revealed its comparable performance to commercial flow cytometer. Furthermore, to demonstrate the credibility of our device, CD4+ lymphocyte cell count of 18.4% in comparison to 19.2% from commercial flow cytometer suggests that this device possesses the potential of a useful POC diagnostic tool.
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M3 - Conference contribution
AN - SCOPUS:84941711357
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 1068
EP - 1070
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -
