Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip

Ahmad Ahsan Nawaz; Ruth Helmus Nissly; Peng Li; Yasir M. Shariff; Lin Wang; Tony Jun Huang

Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip

Ahmad Ahsan Nawaz
, Ruth Helmus Nissly
, Peng Li
, Yasir M. Shariff
, Lin Wang
, Tony Jun Huang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Here we present an integrated, "microfluidic drifting" based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. Our device is capable of analyzing human blood for cell counting and diagnosis of diseases at a throughput of ∼3,754 events/sec. 10 μm Flow-Check Fluorospheres polystyrene (PS) calibration beads (Beckman Coulter, Inc.) were used for characterization of Forward Scatter, Side Scatter, and Fluorescent signals revealing coefficient of variation (CV) of 3.92%, 3.82%, and 9.81%, respectively. Comparison of Immonological analysis of human blood from healthy donor via microfluidic drifting flow cytometry chip revealed its comparable performance to commercial flow cytometer. Furthermore, to demonstrate the credibility of our device, CD4+ lymphocyte cell count of 18.4% in comparison to 19.2% from commercial flow cytometer suggests that this device possesses the potential of a useful POC diagnostic tool.

Original language	English (US)
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	1068-1070
Number of pages	3
ISBN (Electronic)	9780979806476
State	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014

Publication series

Name	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/Territory	United States
City	San Antonio
Period	10/26/14 → 10/30/14

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Control and Systems Engineering

Cite this

Nawaz, A. A., Nissly, R. H., Li, P., Shariff, Y. M., Wang, L., & Huang, T. J. (2014). Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1068-1070). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Nawaz, Ahmad Ahsan ; Nissly, Ruth Helmus ; Li, Peng et al. / Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 1068-1070 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

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title = "Differential analysis of lysed whole blood via {"}Microfluidic drifting{"} based flow cytometry chip",

abstract = "Here we present an integrated, {"}microfluidic drifting{"} based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. Our device is capable of analyzing human blood for cell counting and diagnosis of diseases at a throughput of ∼3,754 events/sec. 10 μm Flow-Check Fluorospheres polystyrene (PS) calibration beads (Beckman Coulter, Inc.) were used for characterization of Forward Scatter, Side Scatter, and Fluorescent signals revealing coefficient of variation (CV) of 3.92\%, 3.82\%, and 9.81\%, respectively. Comparison of Immonological analysis of human blood from healthy donor via microfluidic drifting flow cytometry chip revealed its comparable performance to commercial flow cytometer. Furthermore, to demonstrate the credibility of our device, CD4+ lymphocyte cell count of 18.4\% in comparison to 19.2\% from commercial flow cytometer suggests that this device possesses the potential of a useful POC diagnostic tool.",

author = "Nawaz, \{Ahmad Ahsan\} and Nissly, \{Ruth Helmus\} and Peng Li and Shariff, \{Yasir M.\} and Lin Wang and Huang, \{Tony Jun\}",

note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

year = "2014",

language = "English (US)",

series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

publisher = "Chemical and Biological Microsystems Society",

pages = "1068--1070",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

}

Nawaz, AA, Nissly, RH, Li, P, Shariff, YM, Wang, L & Huang, TJ 2014, Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 1068-1070, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.

Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip. / Nawaz, Ahmad Ahsan; Nissly, Ruth Helmus; Li, Peng et al.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1068-1070 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip

AU - Nawaz, Ahmad Ahsan

AU - Nissly, Ruth Helmus

AU - Li, Peng

AU - Shariff, Yasir M.

AU - Wang, Lin

AU - Huang, Tony Jun

PY - 2014

Y1 - 2014

N2 - Here we present an integrated, "microfluidic drifting" based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. Our device is capable of analyzing human blood for cell counting and diagnosis of diseases at a throughput of ∼3,754 events/sec. 10 μm Flow-Check Fluorospheres polystyrene (PS) calibration beads (Beckman Coulter, Inc.) were used for characterization of Forward Scatter, Side Scatter, and Fluorescent signals revealing coefficient of variation (CV) of 3.92%, 3.82%, and 9.81%, respectively. Comparison of Immonological analysis of human blood from healthy donor via microfluidic drifting flow cytometry chip revealed its comparable performance to commercial flow cytometer. Furthermore, to demonstrate the credibility of our device, CD4+ lymphocyte cell count of 18.4% in comparison to 19.2% from commercial flow cytometer suggests that this device possesses the potential of a useful POC diagnostic tool.

AB - Here we present an integrated, "microfluidic drifting" based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. Our device is capable of analyzing human blood for cell counting and diagnosis of diseases at a throughput of ∼3,754 events/sec. 10 μm Flow-Check Fluorospheres polystyrene (PS) calibration beads (Beckman Coulter, Inc.) were used for characterization of Forward Scatter, Side Scatter, and Fluorescent signals revealing coefficient of variation (CV) of 3.92%, 3.82%, and 9.81%, respectively. Comparison of Immonological analysis of human blood from healthy donor via microfluidic drifting flow cytometry chip revealed its comparable performance to commercial flow cytometer. Furthermore, to demonstrate the credibility of our device, CD4+ lymphocyte cell count of 18.4% in comparison to 19.2% from commercial flow cytometer suggests that this device possesses the potential of a useful POC diagnostic tool.

UR - https://www.scopus.com/pages/publications/84941711357

UR - https://www.scopus.com/pages/publications/84941711357#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:84941711357

T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

SP - 1068

EP - 1070

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

Nawaz AA, Nissly RH, Li P, Shariff YM, Wang L, Huang TJ. Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 1068-1070. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Differential analysis of lysed whole blood via "Microfluidic drifting" based flow cytometry chip

Abstract

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All Science Journal Classification (ASJC) codes

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