Differential destabilization of repetitive sequence hybrids in fluorescence in situ hybridization

J. C. Hozier; J. M. Scalzi; A. C. Clase; L. M. Davis; M. C. Liechty

doi:10.1159/000015127

Differential destabilization of repetitive sequence hybrids in fluorescence in situ hybridization

J. C. Hozier, J. M. Scalzi, A. C. Clase, L. M. Davis, M. C. Liechty

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4 Scopus citations

Abstract

A method for painting a chromosome or chromosome region by fluorescence in situ hybridization (FISH) without blocking DNA is described. Both unique sequence and repetitive sequence components of a fluorescently labeled probe are hybridized under low-stringency conditions, but the chromosomes are washed in such a manner that repetitive sequences are differentially removed, while region-specific unique sequence fragments remain bound to the target chromosomes. We refer to this differential retention and removal of probe components as differential stability FISH.

Original language	English (US)
Pages (from-to)	60-63
Number of pages	4
Journal	Cytogenetics and Cell Genetics
Volume	83
Issue number	1-2
DOIs	https://doi.org/10.1159/000015127
State	Published - 1998

All Science Journal Classification (ASJC) codes

Genetics
Cell Biology

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10.1159/000015127

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title = "Differential destabilization of repetitive sequence hybrids in fluorescence in situ hybridization",

abstract = "A method for painting a chromosome or chromosome region by fluorescence in situ hybridization (FISH) without blocking DNA is described. Both unique sequence and repetitive sequence components of a fluorescently labeled probe are hybridized under low-stringency conditions, but the chromosomes are washed in such a manner that repetitive sequences are differentially removed, while region-specific unique sequence fragments remain bound to the target chromosomes. We refer to this differential retention and removal of probe components as differential stability FISH.",

author = "Hozier, {J. C.} and Scalzi, {J. M.} and Clase, {A. C.} and Davis, {L. M.} and Liechty, {M. C.}",

year = "1998",

doi = "10.1159/000015127",

language = "English (US)",

volume = "83",

pages = "60--63",

journal = "Cytogenetics and Cell Genetics",

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publisher = "S. Karger AG",

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T1 - Differential destabilization of repetitive sequence hybrids in fluorescence in situ hybridization

AU - Hozier, J. C.

AU - Scalzi, J. M.

AU - Clase, A. C.

AU - Davis, L. M.

AU - Liechty, M. C.

PY - 1998

Y1 - 1998

N2 - A method for painting a chromosome or chromosome region by fluorescence in situ hybridization (FISH) without blocking DNA is described. Both unique sequence and repetitive sequence components of a fluorescently labeled probe are hybridized under low-stringency conditions, but the chromosomes are washed in such a manner that repetitive sequences are differentially removed, while region-specific unique sequence fragments remain bound to the target chromosomes. We refer to this differential retention and removal of probe components as differential stability FISH.

AB - A method for painting a chromosome or chromosome region by fluorescence in situ hybridization (FISH) without blocking DNA is described. Both unique sequence and repetitive sequence components of a fluorescently labeled probe are hybridized under low-stringency conditions, but the chromosomes are washed in such a manner that repetitive sequences are differentially removed, while region-specific unique sequence fragments remain bound to the target chromosomes. We refer to this differential retention and removal of probe components as differential stability FISH.

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U2 - 10.1159/000015127

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JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

IS - 1-2

ER -

Differential destabilization of repetitive sequence hybrids in fluorescence in situ hybridization

Abstract

All Science Journal Classification (ASJC) codes

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