Abstract
A method for painting a chromosome or chromosome region by fluorescence in situ hybridization (FISH) without blocking DNA is described. Both unique sequence and repetitive sequence components of a fluorescently labeled probe are hybridized under low-stringency conditions, but the chromosomes are washed in such a manner that repetitive sequences are differentially removed, while region-specific unique sequence fragments remain bound to the target chromosomes. We refer to this differential retention and removal of probe components as differential stability FISH.
Original language | English (US) |
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Pages (from-to) | 60-63 |
Number of pages | 4 |
Journal | Cytogenetics and Cell Genetics |
Volume | 83 |
Issue number | 1-2 |
DOIs | |
State | Published - 1998 |
All Science Journal Classification (ASJC) codes
- Genetics
- Cell Biology