Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas

John W. Lin, Andrew V. Biankin, Marko E. Horb, Bidyut Ghosh, Nijaguna B. Prasad, Nelson S. Yee, Michael A. Pack, Steven D. Leach

Research output: Contribution to journalArticlepeer-review

65 Scopus citations


Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor Ptf1a-p48 in the development of both exocrine and endocrine pancreas. We have cloned the Ptf1a-p48 ortholog in Danio rerio. Early zebrafish ptf1a expression is observed in developing hindbrain and in endodermal pancreatic precursors. Analysis of ptf1a and insulin expression reveals a population of exocrine precursors that, throughout early development, are temporally and spatially segregated from endocrine elements. Morpholino-mediated knockdown of ptf1a confirms early divergence of these endocrine and exocrine lineages. Ptf1a morphants lack differentiated exocrine pancreas, but maintain normal differentiation and organization of the principal islet. In addition to the exocrine phenotype, ptf1a knockdown also reduces the prevalence of a small population of anterior endocrine cells normally found outside the principal islet. Together, these findings suggest the presence of distinct ptf1a-dependent and ptf1a-independent precursor populations in developing zebrafish pancreas.

Original languageEnglish (US)
Pages (from-to)474-486
Number of pages13
JournalDevelopmental biology
Issue number2
StatePublished - Jun 15 2004

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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