TY - JOUR
T1 - Differentiation of y79 retinoblastoma cells with pigment epithelial-derived factor and interphotoreceptor matrix wash
T2 - Effects on tumorigenicity
AU - Seigel, Gail M.
AU - Tombran-Tink, Joyce
AU - Becerra, S. Patricia
AU - Chader, Gerald J.
AU - Diloreto, David A.
AU - Cerro, Constancia Del
AU - Lazar, Eliot S.
AU - Cerro, Manuel Del
N1 - Funding Information:
This research was supported, in part, by NIH#05262, Lucille P. Markey Charitable Trust Fund to the University of Rochester (G.M.S.), The Rochester Eye Bank, and private gifts.
PY - 1994
Y1 - 1994
N2 - We investigated the in vivo differentiation potential of Y79 human retinoblastoma cells following pre-treatment with two novel neurotrophic agents: PEDF (human recombinant pigmented-epithelial derived factor) or IPM (interphotoreceptor matrix) wash. These agents were able to induce a significant degree of morphological differentiation in vitro. However, 48 days after subretinal transplantation of pre-treated cells, massive tumor formation was apparent. In contrast, Y79 cells pre-treated with retinoic acid/sodium butyrate, which attain a lesser degree of morphological differentiation, did not produce tumors over a 30 to 60 day-survival time (del Cerro et al., Brain Research, 12-22, 1992). We conclude that for PEDF and IPM, the degree of in vitro differentiation and the degree of mitotic arrest are independent features.
AB - We investigated the in vivo differentiation potential of Y79 human retinoblastoma cells following pre-treatment with two novel neurotrophic agents: PEDF (human recombinant pigmented-epithelial derived factor) or IPM (interphotoreceptor matrix) wash. These agents were able to induce a significant degree of morphological differentiation in vitro. However, 48 days after subretinal transplantation of pre-treated cells, massive tumor formation was apparent. In contrast, Y79 cells pre-treated with retinoic acid/sodium butyrate, which attain a lesser degree of morphological differentiation, did not produce tumors over a 30 to 60 day-survival time (del Cerro et al., Brain Research, 12-22, 1992). We conclude that for PEDF and IPM, the degree of in vitro differentiation and the degree of mitotic arrest are independent features.
UR - https://www.scopus.com/pages/publications/0028065015
UR - https://www.scopus.com/pages/publications/0028065015#tab=citedBy
U2 - 10.3109/08977199409010995
DO - 10.3109/08977199409010995
M3 - Article
C2 - 7803045
AN - SCOPUS:0028065015
SN - 0897-7194
VL - 10
SP - 289
EP - 297
JO - Growth Factors
JF - Growth Factors
IS - 4
ER -