@inproceedings{782f12a5fb8548b6b1963661ff19c879,
title = "Digital CRISPR-based quantification of HIV-1",
abstract = "Here, we demonstrate a membrane-based digital CRISPR-Cas13a system for amplification-free absolute quantification of viral particles of HIV-1. We established a stamping technique to digitalize the Cas13 assay inside a commercial track-etched polycarbonate (PCTE) membrane. We evaluated the performance of our system by quantifying the synthetic HIV-1 RNA, where a limit of detection of 100 aM was achieved in 30 min reaction. Absolute quantification of the viral particles HIV-1 in plasma background using our system confirmed that our system can quantify spiked samples.",
author = "Reza Nouri and Yuqian Jiang and Politza, {Anthony J.} and Lian, {Xiaojun Lance} and Weihua Guan",
note = "Publisher Copyright: {\textcopyright} 2022 IEEE.; 2022 IEEE Sensors Conference, SENSORS 2022 ; Conference date: 30-10-2022 Through 02-11-2022",
year = "2022",
doi = "10.1109/SENSORS52175.2022.9967104",
language = "English (US)",
series = "Proceedings of IEEE Sensors",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
booktitle = "2022 IEEE Sensors, SENSORS 2022 - Conference Proceedings",
address = "United States",
}