Digital CRISPR-based quantification of HIV-1

Reza Nouri, Yuqian Jiang, Anthony J. Politza, Xiaojun Lance Lian, Weihua Guan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here, we demonstrate a membrane-based digital CRISPR-Cas13a system for amplification-free absolute quantification of viral particles of HIV-1. We established a stamping technique to digitalize the Cas13 assay inside a commercial track-etched polycarbonate (PCTE) membrane. We evaluated the performance of our system by quantifying the synthetic HIV-1 RNA, where a limit of detection of 100 aM was achieved in 30 min reaction. Absolute quantification of the viral particles HIV-1 in plasma background using our system confirmed that our system can quantify spiked samples.

Original languageEnglish (US)
Title of host publication2022 IEEE Sensors, SENSORS 2022 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781665484640
DOIs
StatePublished - 2022
Event2022 IEEE Sensors Conference, SENSORS 2022 - Dallas, United States
Duration: Oct 30 2022Nov 2 2022

Publication series

NameProceedings of IEEE Sensors
Volume2022-October
ISSN (Print)1930-0395
ISSN (Electronic)2168-9229

Conference

Conference2022 IEEE Sensors Conference, SENSORS 2022
Country/TerritoryUnited States
CityDallas
Period10/30/2211/2/22

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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