Direct Mapping of Phospholipid Ferroptotic Death Signals in Cells and Tissues by Gas Cluster Ion Beam Secondary Ion Mass Spectrometry (GCIB-SIMS)

Louis J. Sparvero; Hua Tian; Andrew A. Amoscato; Wan Yang Sun; Tamil S. Anthonymuthu; Yulia Y. Tyurina; Oleksandr Kapralov; Sabzali Javadov; Rong Rong He; Simon C. Watkins; Nicholas Winograd; Valerian E. Kagan; Hülya Bayır

doi:10.1002/anie.202102001

Direct Mapping of Phospholipid Ferroptotic Death Signals in Cells and Tissues by Gas Cluster Ion Beam Secondary Ion Mass Spectrometry (GCIB-SIMS)

Louis J. Sparvero, Hua Tian, Andrew A. Amoscato, Wan Yang Sun, Tamil S. Anthonymuthu, Yulia Y. Tyurina, Oleksandr Kapralov, Sabzali Javadov, Rong Rong He, Simon C. Watkins, Nicholas Winograd, Valerian E. Kagan, Hülya Bayır

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Peroxidized phosphatidylethanolamine (PEox) species have been identified by liquid chromatography mass spectrometry (LC-MS) as predictive biomarkers of ferroptosis, a new program of regulated cell death. However, the presence and subcellular distribution of PEox in specific cell types and tissues have not been directly detected by imaging protocols. By applying gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) imaging with a 70 keV (H₂O)_n⁺ (n>28 000) cluster ion beam, we were able to map PEox with 1.2 μm spatial resolution at the single cell/subcellular level in ferroptotic H9c2 cardiomyocytes and cortical/hippocampal neurons after traumatic brain injury. Application of this protocol affords visualization of physiologically relevant levels of very low abundance (20 pmol μmol⁻¹ lipid) peroxidized lipids in subcellular compartments and their accumulation in disease conditions.

Original language	English (US)
Pages (from-to)	11784-11788
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	60
Issue number	21
DOIs	https://doi.org/10.1002/anie.202102001
State	Published - May 17 2021

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry

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10.1002/anie.202102001

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Sparvero, L. J., Tian, H., Amoscato, A. A., Sun, W. Y., Anthonymuthu, T. S., Tyurina, Y. Y., Kapralov, O., Javadov, S., He, R. R., Watkins, S. C., Winograd, N., Kagan, V. E., & Bayır, H. (2021). Direct Mapping of Phospholipid Ferroptotic Death Signals in Cells and Tissues by Gas Cluster Ion Beam Secondary Ion Mass Spectrometry (GCIB-SIMS). Angewandte Chemie - International Edition, 60(21), 11784-11788. https://doi.org/10.1002/anie.202102001

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title = "Direct Mapping of Phospholipid Ferroptotic Death Signals in Cells and Tissues by Gas Cluster Ion Beam Secondary Ion Mass Spectrometry (GCIB-SIMS)",

abstract = "Peroxidized phosphatidylethanolamine (PEox) species have been identified by liquid chromatography mass spectrometry (LC-MS) as predictive biomarkers of ferroptosis, a new program of regulated cell death. However, the presence and subcellular distribution of PEox in specific cell types and tissues have not been directly detected by imaging protocols. By applying gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) imaging with a 70 keV (H2O)n+ (n>28 000) cluster ion beam, we were able to map PEox with 1.2 μm spatial resolution at the single cell/subcellular level in ferroptotic H9c2 cardiomyocytes and cortical/hippocampal neurons after traumatic brain injury. Application of this protocol affords visualization of physiologically relevant levels of very low abundance (20 pmol μmol−1 lipid) peroxidized lipids in subcellular compartments and their accumulation in disease conditions.",

author = "Sparvero, {Louis J.} and Hua Tian and Amoscato, {Andrew A.} and Sun, {Wan Yang} and Anthonymuthu, {Tamil S.} and Tyurina, {Yulia Y.} and Oleksandr Kapralov and Sabzali Javadov and He, {Rong Rong} and Watkins, {Simon C.} and Nicholas Winograd and Kagan, {Valerian E.} and H{\"u}lya Bayır",

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year = "2021",

month = may,

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doi = "10.1002/anie.202102001",

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Sparvero, LJ, Tian, H, Amoscato, AA, Sun, WY, Anthonymuthu, TS, Tyurina, YY, Kapralov, O, Javadov, S, He, RR, Watkins, SC, Winograd, N, Kagan, VE & Bayır, H 2021, 'Direct Mapping of Phospholipid Ferroptotic Death Signals in Cells and Tissues by Gas Cluster Ion Beam Secondary Ion Mass Spectrometry (GCIB-SIMS)', Angewandte Chemie - International Edition, vol. 60, no. 21, pp. 11784-11788. https://doi.org/10.1002/anie.202102001

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AU - Sparvero, Louis J.

AU - Tian, Hua

AU - Amoscato, Andrew A.

AU - Sun, Wan Yang

AU - Anthonymuthu, Tamil S.

AU - Tyurina, Yulia Y.

AU - Kapralov, Oleksandr

AU - Javadov, Sabzali

AU - He, Rong Rong

AU - Watkins, Simon C.

AU - Winograd, Nicholas

AU - Kagan, Valerian E.

AU - Bayır, Hülya

PY - 2021/5/17

Y1 - 2021/5/17

N2 - Peroxidized phosphatidylethanolamine (PEox) species have been identified by liquid chromatography mass spectrometry (LC-MS) as predictive biomarkers of ferroptosis, a new program of regulated cell death. However, the presence and subcellular distribution of PEox in specific cell types and tissues have not been directly detected by imaging protocols. By applying gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) imaging with a 70 keV (H2O)n+ (n>28 000) cluster ion beam, we were able to map PEox with 1.2 μm spatial resolution at the single cell/subcellular level in ferroptotic H9c2 cardiomyocytes and cortical/hippocampal neurons after traumatic brain injury. Application of this protocol affords visualization of physiologically relevant levels of very low abundance (20 pmol μmol−1 lipid) peroxidized lipids in subcellular compartments and their accumulation in disease conditions.

AB - Peroxidized phosphatidylethanolamine (PEox) species have been identified by liquid chromatography mass spectrometry (LC-MS) as predictive biomarkers of ferroptosis, a new program of regulated cell death. However, the presence and subcellular distribution of PEox in specific cell types and tissues have not been directly detected by imaging protocols. By applying gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) imaging with a 70 keV (H2O)n+ (n>28 000) cluster ion beam, we were able to map PEox with 1.2 μm spatial resolution at the single cell/subcellular level in ferroptotic H9c2 cardiomyocytes and cortical/hippocampal neurons after traumatic brain injury. Application of this protocol affords visualization of physiologically relevant levels of very low abundance (20 pmol μmol−1 lipid) peroxidized lipids in subcellular compartments and their accumulation in disease conditions.

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Direct Mapping of Phospholipid Ferroptotic Death Signals in Cells and Tissues by Gas Cluster Ion Beam Secondary Ion Mass Spectrometry (GCIB-SIMS)

Abstract

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