TY - JOUR
T1 - Distinguishing Förster resonance energy transfer and solvent-mediated charge-transfer relaxation dynamics in a zinc(ii) indicator
T2 - A femtosecond time-resolved transient absorption spectroscopic study
AU - Sreenath, Kesavapillai
AU - Yi, Chongyue
AU - Knappenberger, Kenneth L.
AU - Zhu, Lei
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/3/21
Y1 - 2014/3/21
N2 - A bifluorophoric molecule (1) capable of intramolecular Förster Resonance Energy Transfer (FRET) is reported. The emission intensity of the FRET acceptor in 1 depends on the molar absorptivity of the donor, which is a function of zinc(ii) complexation. The FRET dynamics of [Zn(1)](ClO 4)2 is characterized by femtosecond time-resolved transient absorption spectroscopy. The solvent-mediated relaxation of the charge-transfer (CT) state of the isolated donor and the FRET process of the donor-acceptor conjugate are on similar time scales (40-50 ps in CH 3CN), but distinguishable by the opposite solvent polarity dependency. As the solvent polarity increases, the efficiency of Columbic-based FRET is reduced, whereas CT relaxation is accelerated. In addition to revealing a method to distinguish CT and FRET dynamics, this work provides a photophysical foundation for developing indicators based on the FRET strategy.
AB - A bifluorophoric molecule (1) capable of intramolecular Förster Resonance Energy Transfer (FRET) is reported. The emission intensity of the FRET acceptor in 1 depends on the molar absorptivity of the donor, which is a function of zinc(ii) complexation. The FRET dynamics of [Zn(1)](ClO 4)2 is characterized by femtosecond time-resolved transient absorption spectroscopy. The solvent-mediated relaxation of the charge-transfer (CT) state of the isolated donor and the FRET process of the donor-acceptor conjugate are on similar time scales (40-50 ps in CH 3CN), but distinguishable by the opposite solvent polarity dependency. As the solvent polarity increases, the efficiency of Columbic-based FRET is reduced, whereas CT relaxation is accelerated. In addition to revealing a method to distinguish CT and FRET dynamics, this work provides a photophysical foundation for developing indicators based on the FRET strategy.
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U2 - 10.1039/c3cp55382e
DO - 10.1039/c3cp55382e
M3 - Article
AN - SCOPUS:84894480510
SN - 1463-9076
VL - 16
SP - 5088
EP - 5092
JO - Physical Chemistry Chemical Physics
JF - Physical Chemistry Chemical Physics
IS - 11
ER -