TY - JOUR
T1 - Distribution of Guinea Pig Herpeslike Virus in Brain and Other Tissues of Naturally and Experimentally Infected Animals
AU - Tenser, R. B.
N1 - Funding Information:
This work was supported by USPHS Research Grant No. AI-08648 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland. The excellent technical assitance of Mrs. K. Hastings and Mrs. R. Straus is gratefully acknowledged, as is the clerical assistance of Mrs. V. Mercadante. 2
Funding Information:
Recipient of Research Fellowship No. 1 F22 NS00873-01 from the National Institute of Neurological and Communicative Disorders and Stroke (NINCDS).
PY - 1976/7
Y1 - 1976/7
N2 - Distribution of GPHLV in the trigeminal ganglion, brain, and other tissues of guinea pigs was studied. Virus was found in suspensions of all tissue cells from naturally infected guinea pigs, as well as animals inoculated ip or ic, up to 15 months after initiation of infection. Evidence of virus infection was detected by cultivation of cells or by cocultivation of infected cells with susceptible cell cultures, but not by other methods including immunofluorescence and electron microscopy. Quantitative determinations of virus in the trigeminal ganglia and different parts of the brain showed infectivity titers to be similar in these tissues. Since GPHLV was also found in blood and in high titer in spleen, and in other tissues tested, isolation of virus from neural tissues may have been due to contamination with infected blood leukocytes.
AB - Distribution of GPHLV in the trigeminal ganglion, brain, and other tissues of guinea pigs was studied. Virus was found in suspensions of all tissue cells from naturally infected guinea pigs, as well as animals inoculated ip or ic, up to 15 months after initiation of infection. Evidence of virus infection was detected by cultivation of cells or by cocultivation of infected cells with susceptible cell cultures, but not by other methods including immunofluorescence and electron microscopy. Quantitative determinations of virus in the trigeminal ganglia and different parts of the brain showed infectivity titers to be similar in these tissues. Since GPHLV was also found in blood and in high titer in spleen, and in other tissues tested, isolation of virus from neural tissues may have been due to contamination with infected blood leukocytes.
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U2 - 10.3181/00379727-152-39412
DO - 10.3181/00379727-152-39412
M3 - Article
C2 - 181762
AN - SCOPUS:0017117665
SN - 0037-9727
VL - 152
SP - 428
EP - 431
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 3
ER -