Dithiothreitol and amino acids interfere with the fluorometric determination of glutathione with orthophthaldehyde

Russell C. Scaduto

doi:10.1016/0003-2697(88)90544-1

Dithiothreitol and amino acids interfere with the fluorometric determination of glutathione with orthophthaldehyde

Russell C. Scaduto

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19 Scopus citations

Abstract

The reaction of orthophthaldehyde with glutathione at pH 8.0 is commonly employed as a specific fluorometric assay for glutathione. However, this method has been reported to overestimate the glutathione concentration when compared with either enzymatic or HPLC-based methods. We have found that amino acids and other thiols interfere in the reaction of glutathione with orthophthaldehyde, causing erroneous estimates of glutathione concentration. The extent of this interference is described.

Original language	English (US)
Pages (from-to)	265-270
Number of pages	6
Journal	Analytical Biochemistry
Volume	174
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(88)90544-1
State	Published - Oct 1988

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(88)90544-1

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title = "Dithiothreitol and amino acids interfere with the fluorometric determination of glutathione with orthophthaldehyde",

abstract = "The reaction of orthophthaldehyde with glutathione at pH 8.0 is commonly employed as a specific fluorometric assay for glutathione. However, this method has been reported to overestimate the glutathione concentration when compared with either enzymatic or HPLC-based methods. We have found that amino acids and other thiols interfere in the reaction of glutathione with orthophthaldehyde, causing erroneous estimates of glutathione concentration. The extent of this interference is described.",

author = "Scaduto, \{Russell C.\}",

year = "1988",

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AU - Scaduto, Russell C.

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N2 - The reaction of orthophthaldehyde with glutathione at pH 8.0 is commonly employed as a specific fluorometric assay for glutathione. However, this method has been reported to overestimate the glutathione concentration when compared with either enzymatic or HPLC-based methods. We have found that amino acids and other thiols interfere in the reaction of glutathione with orthophthaldehyde, causing erroneous estimates of glutathione concentration. The extent of this interference is described.

AB - The reaction of orthophthaldehyde with glutathione at pH 8.0 is commonly employed as a specific fluorometric assay for glutathione. However, this method has been reported to overestimate the glutathione concentration when compared with either enzymatic or HPLC-based methods. We have found that amino acids and other thiols interfere in the reaction of glutathione with orthophthaldehyde, causing erroneous estimates of glutathione concentration. The extent of this interference is described.

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U2 - 10.1016/0003-2697(88)90544-1

DO - 10.1016/0003-2697(88)90544-1

M3 - Article

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AN - SCOPUS:0023779514

SN - 0003-2697

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Dithiothreitol and amino acids interfere with the fluorometric determination of glutathione with orthophthaldehyde

Abstract

All Science Journal Classification (ASJC) codes

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