TY - JOUR
T1 - DNA binding-induced conformational change of the yeast transcriptional activator PRTF
AU - Tan, Song
AU - Richmond, Timothy J.
N1 - Funding Information:
We are very grateful to Gustav Ammerer for his generous gift of purified PRTF and thank Beverly Errede and Gustav Ammerer for communicating results prior to publication. We also would like to thank Fritz Thoma for critical reading of the manuscript and to one of the referees for comments and suggestions. S. T. thanks Edgar Schreiber, Rene Brunisholz, and Peter K&rig for stimulating discussions. S. T. was supported in part by a US. National Science Foundation graduate fellowship.
PY - 1990/7/27
Y1 - 1990/7/27
N2 - Our studies using proteases to probe protein structure establish that binding to the upstream activating sequences (UASs) of two different yeast a-specific genes induces a conformational change in the pheromone/receptor transcription factor (PRTF), which is not observed upon binding to the UASs of either of two α-specific genes. We propose that this selective structural alteration exposes an activation region of PRTF when it binds a-specific genes, switching these genes on. The transcriptional activator MATα1 may activate α-specific genes by binding to the PRTF-α-specific UAS complex and unmasking the otherwise hidden activation surface of PRTF. We also show that the N-terminal third of PRTF is sufficient for specific DNA binding, while the middle third of the protein interacts with MATα1.
AB - Our studies using proteases to probe protein structure establish that binding to the upstream activating sequences (UASs) of two different yeast a-specific genes induces a conformational change in the pheromone/receptor transcription factor (PRTF), which is not observed upon binding to the UASs of either of two α-specific genes. We propose that this selective structural alteration exposes an activation region of PRTF when it binds a-specific genes, switching these genes on. The transcriptional activator MATα1 may activate α-specific genes by binding to the PRTF-α-specific UAS complex and unmasking the otherwise hidden activation surface of PRTF. We also show that the N-terminal third of PRTF is sufficient for specific DNA binding, while the middle third of the protein interacts with MATα1.
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U2 - 10.1016/0092-8674(90)90373-M
DO - 10.1016/0092-8674(90)90373-M
M3 - Article
C2 - 2164891
AN - SCOPUS:0025363427
SN - 0092-8674
VL - 62
SP - 367
EP - 377
JO - Cell
JF - Cell
IS - 2
ER -